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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Inactivation of plant-pathogenic fungus Colletotrichum acutatum with natural plant-produced photosensitizers under solar radiation

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Autor(es):
Fracarolli, Leticia ; Rodrigues, Gabriela B. ; Pereira, Ana C. ; Massola Junior, Nelson S. ; Silva-Junior, Geraldo Jose ; Bachmann, Luciano ; Wainwright, Mark ; Bastos, Jairo Kenupp ; Braga, Gilberto U. L.
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 162, p. 402-411, SEP 2016.
Citações Web of Science: 10
Resumo

The increasing tolerance to currently used fungicides and the need for environmentally friendly antimicrobial approaches have stimulated the development of novel strategies to control plant-pathogenic fungi such as antimicrobial phototreatment (APT). We investigated the in vitro APT of the plant-pathogenic fungus Colletotrichum acutatum with furocoumarins and coumarins and solar radiation. The compounds used were: furocoumarins 8-methoxypsoralen (8-MOP) and 5,8-dimethoxypsoralen (isopimpinellin), coumarins 2H-chromen-2-one (coumarin), 7-hydroxycoumarin, 5,7-dimethoxycoumarin (citropten) and a mixture (3:1) of 7-methoxycoumarin and 5,7-dimethoxycoumarin. APT of conidia with crude extracts from `Tahiti' acid lime, red and white grapefruit were also performed. Pure compounds were tested at 50 mu M concentration and mixtures and extracts at 12.5 mg L-1. The C. acutatum conidia suspension with or without the compounds was exposed to solar radiation for 1 h. In addition, the effects of APT on the leaves of the plant host Citrus sinensis were determined. APT with 8 MOP was the most effective treatment, killing 100% of the conidia followed by the mixture of two coumarins and isopimpinellin that killed 99% and 64% of the conidia, respectively. APT with the extracts killed from 20% to 70% of the conidia, and the extract from `Tahiti' lime was the most effective. No damage to sweet orange leaves was observed after APT with any of the compounds or extracts. (C) 2016 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 12/15204-8 - Estudo mecanístico da inativação fotodinâmica de fungos patógenos de humanos e de fungos fitopatógenos
Beneficiário:Gilberto Úbida Leite Braga
Modalidade de apoio: Auxílio à Pesquisa - Regular