Infection of mice with oocysts of Toxoplasma gondii by oral route showed differences of virulence from Brazilian RFLP genotypes BrI and BrIII

Autor(es)

Chiebao, Daniela Pontes ; de Jesus Pena, Hilda Fatima ; Cabral, Aline Diniz ; Rocca, Mayra Pereira ; Lopes, Estela Gallucci ; Oshiro Branco Valadas, Samantha Yuri ; Keid, Lara Borges ; Hildebrand Grisi Filho, Jose Henrique ; Soares, Rodrigo Martins

• Tipo de documento: Artigo Científico
• Fonte: Research in Veterinary Science; v. 107, p. 257-260, AUG 2016.
• Citações Web of Science: 3

Resumo

South American strains of Toxoplasma gondii present higher genetic diversity than classical European strains. We compared the virulence of two non-archetypal Brazilian genotypes of T. gondii to mice. Oocysts of four isolates, two genotype BrI (TgCatBr71 and TgShBr11) and two Brill (TgCatBr74 and TgCatBr60) were obtained from cats fed experimentally infected mice. After sporulation, 5.0 x 10(1) and 1.0 x 10(2) oocysts were orally administrated to Swiss albine mice in Experiments \#1 and \#2, respectively (4-10 mice/group). Humoral response from dead and surviving mice was analyzed on days 9 to 35 post-infection. Microscopic observations of lungs and brains were performed for tachyzoites and cysts visualization in fresh preparations. Negative results were tested by PCR Virulence after infection with oocysts is dose dependent for genotype Brill isolates, but not for BrI. Differences in mortality were observed among isolates from genotype BrIII on Experiment \#1. Intra-genotype phenotypic variation related to the parasite stage of infection was demonstrated and this characteristic should be further studied and may influence future work regarding the role of virulence amid hosts. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/19297-8 - Estudo da infecção oral por Toxoplasma gondii em ovinos: avaliação da transmissão congênita em infecções experimentais por meio de diferentes linhagens brasileiras
• Beneficiário: Rodrigo Martins Soares
• Modalidade de apoio: Auxílio à Pesquisa - Regular