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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae

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de Souza, Cristiane S. ; Torres, Alfredo G. ; Caravelli, Andressa ; Silva, Anderson ; Polatto, Juliana M. ; Piazza, Roxane M. F.
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: Protein Science; v. 25, n. 12, p. 2142-2151, DEC 2016.
Citações Web of Science: 4

Atypical enteropathogenic Escherichia coli (aEPEC) are heterogeneous strains in terms of serotypes, adherence patterns and the presence of novel virulence factors. This heterogeneity is intriguing, promoting studies trying to characterize these novel proteins and to better comprehend this pathotype group. In a previous study analyzing low-molecular mass proteomes of four representative aEPEC strains of three different adhesion phenotypes, we classified proteins according to their annotated function, with most of them being involved in metabolism and transport; while some of them were classified as hypothetical proteins. The majority of the hypothetical proteins were homologue products of genes identified in the genome of enterohemorrhagic E. coli. One of the hypothetical proteins was annotated as Z2335, with orthologue in EPEC, and by bioinformatics analysis, this protein was revealed to be the universal stress protein F (UspF). Thus, herein we successfully obtained a recombinant UspF protein from aEPEC, which is a alpha/beta, ATP-binding protein involved in stress response, with comparable protein production among the four studied strains, but showing noteworthy differences when cultivated in different stress conditions, also present in other enterobacterial species, such as Shigella sonnei and Citrobacter freundii. Furthermore, our results confirm that the Usp protein superfamily encompasses a conserved group of proteins involved in stress resistance in aEPEC and other Enterobacteriaceae. (AU)

Processo FAPESP: 09/14845-7 - Imunodiagnóstico de Escherichia coli diarreiogênica
Beneficiário:Roxane Maria Fontes Piazza
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/09206-2 - Clonagem, expressão e caracterização de proteínas de Escherichia coli enteropatogênica atípica (EPEC atípica) do sorotipo O55:H7
Beneficiário:Cristiane Santos de Souza
Linha de fomento: Bolsas no Brasil - Pós-Doutorado