Increased glutathione levels contribute to the beneficial effects of hydrogen sulfide and inducible nitric oxide inhibition in allergic lung inflammation

Autor(es)

Campos, Daiana ; Ravagnani, Felipe G. ; Gurgueira, Sonia A. ; Vercesi, Anibal E. ; Teixeira, Simone A. ; Costa, Soraia K. P. ; Muscara, Marcelo N. ; Ferreira, Heloisa H. A.

• Tipo de documento: Artigo Científico
• Fonte: International Immunopharmacology; v. 39, p. 57-62, OCT 2016.
• Citações Web of Science: 10

Resumo

Objective: The interaction between nitric oxide (NO) and hydrogen sulfide (H2S) in the airways could have significant implications for the pathogenesis and therapeutic effects of both on lung diseases. In this study we investigated whether the beneficial effects of H2S on asthma could be comparable to that inhibition of inducible NO synthase (iNOS). Methods: Female BALB/C mice sensitized with ovalbumin (OVA) received either the H2S donor sodium hydrosulfide (NaHS, 14 mu mol/kg) or the iNOS inhibitor 1400W (1 mg/kg), 30 min before each OVA challenge during six days. On the first, second and sixth days, the leucocyte infiltration in lung parenchyma and bronchoalveolar lavage was evaluated. The aconitase activity (a sensor of O-2(center dot-) formation) and lipid peroxidation, as well as levels of reduced glutathione (GSH) and oxidized glutathione (GSSG) were determined in the lung tissues. Results: OVA-challenge caused a significant and time-dependent increase in the eosinophil number in the airways, which was accompanied by a significant decrease of aconitase activity and GSH/GSSG ratio along with enhanced lipid peroxidation in the lungs. Treatment with NaHS or 1400 W significantly attenuated the airways eosinophilia that was paralleled by an increase in aconitase activity and decrease of lipid peroxidation. NaHS or 1400 W treatments also reversed the decreased GSH/GSSG ratio seen after OVA-challenge. Conclusions: The present study shows for the first time that the increased GSH/GSSG ratio caused by either H2S supplementation or iNOS-inhibition is a potential mechanism protecting airways against oxidative stress and inflammatory lung diseases. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/24518-1 - Papel do sulfeto de hidrogênio (H2S) nas respostas inflamatória e nociceptiva induzidas por carragenina na articulação temporomandibular de ratos
• Beneficiário: Marcelo Nicolas Muscara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/02145-3 - Efeito do sulfeto de hidrogênio (H2S) no remodelamento das vias aéreas, na apoptose de eosinófilos e nos níveis de citocinas na inflamação alérgica pulmonar em camundongos
• Beneficiário: Heloisa Helena de Araujo Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/04151-3 - Avaliação farmacológica dos doadores de H2S (de liberação lenta e rápida) no comportamento de prurido e inflamação relacionada em pele de camundongos
• Beneficiário: Soraia Katia Pereira Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular