Enhanced pro-coagulant hemostatic agents based on nanometric zeolites

Autor(es)

Laurenti, Juliana Bergamasco ; Zazeri, Gabriel ; Ribeiro Povinelli, Ana Paula ; de Godoy, Moacir Fernandes ; Braile, Domingo Marcolino ; Flores da Rocha, Tania R. ; D' Amico, Elbio Antonio ; Nery, Jose Geraldo

• Tipo de documento: Artigo Científico
• Fonte: Microporous and Mesoporous Materials; v. 239, p. 263-271, FEB 2017.
• Citações Web of Science: 6

Resumo

Micro-and nanometric faujasite zeolites were tested as hemostatic coagulant agents. The as-made zeolites and their calcium ion exchanged derivatives were characterized by XRD, SEM, AFM, TGA, and DSC. Zeta potential measurements of the micro and nanometric zeolites, as a function of pH (1-12.5), revealed the hemostatic potential of the materials. The isoelectric point for FAU, FAU/Ca, Nano-FAU, and Nano-FAU/Ca were measured at pH 2.0, 1.9, 3.2, and 2.5, respectively. The hemostatic activity was confirmed by the TEG technique for both micro and nanometric zeolites, however a superior activity were observed for the nanometric materials. FAU and FAU/Ca exhibited a reduction of the R parameter (defined as period of time of latency from the start of test to initial fibrin formation for the formation of a clot of an amplitude of 2 mm) from 8.6 +/- 0.7 min (control) to 3.2 +/- 0.7 min and 23 +/- 0.1 min, respectively. On the other hand, for the Nano-FAU, and Nano-FAU/Ca the observed decrease were to 2.4 +/- 0.6 min, and 1.1 +/- 0.2 min, respectively. The amounts of heat released were 105.60 J/g (FAU), 65.8 J/g (FAU/Ca), 85.48 J/g (Nano-FAU), and 78.21 J/g (Nano-FAU/Ca). Statistical analysis using one-way analysis of variance showed a global value of p < 0.0001. Tukey-Kramer multiple comparisons revealed significant (p < 0.0001) differences for FAU, FAU/Ca, Nano-FAU, and Nano-FAU/Ca, relative to the control. Nano-FAU/Ca showed the most important reductions of the R with significant differences compared to the control (p < 0.0001 and p < 0.0001, respectively) and FAU (p = 0.0071 for the R parameter). (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 13/16754-4 - Síntese de agentes hemostáticos coagulantes baseados em novos materiais zeolitícos
• Beneficiário: Juliana Bergamasco Laurenti
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/24259-0 - Moléculas conjugadas peptídeo/quitosana com potencial farmacológico: síntese, prospecção de atividade em sistemas miméticos de membrana e avaliações em células
• Beneficiário: Marcia Perez dos Santos Cabrera
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores