A Novel Carbohydrate-binding Module from Sugar Cane Soil Metagenome Featuring Unique Structural and Carbohydrate Affinity Properties

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Campos, Bruna Medeia ; Liberato, Marcelo Vizona ; Alvarez, Thabata Maria ; Zanphorlin, Leticia Maria ; Ematsu, Gabriela Cristina ; Barud, Hernane ; Polikarpov, Igor ; Ruller, Roberto ; Gilbert, Harry J. ; de Mattos Zeri, Ana Carolina ; Squina, Fabio Marcio

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biological Chemistry; v. 291, n. 45, p. 23734-23743, NOV 4 2016.
• Citações Web of Science: 5

Resumo

Carbohydrate-binding modules (CBMs) are appended to glycoside hydrolases and can contribute to the degradation of complex recalcitrant substrates such as the plant cell wall. For application in bioethanol production, novel enzymes with high catalytic activity against recalcitrant lignocellulosic material are being explored and developed. In this work, we report the functional and structural study of CBM\_E1, which was discovered through a metagenomics approach and is the founding member of a novel CBM family, CBM81. CBM\_E1, which is linked to an endoglucanase, displayed affinity for mixed linked 1,3-1,4-glucans, xyloglucan, Avicel, and cellooligosaccharides. The crystal structure of CBM\_E1 in complex with cellopentaose displayed a canonical -sandwich fold comprising two -sheets. The planar ligand binding site, observed in a parallel orientation with the -strands, is a typical feature of type A CBMs, although the expected affinity for bacterial crystalline cellulose was not detected. Conversely, the binding to soluble glucans was enthalpically driven, which is typical of type B modules. These unique properties of CBM\_E1 are at the interface between type A and type B CBMs. (AU)

• Processo FAPESP: 13/06336-0 - Estudo das Bases Moleculares de Módulos de Ligação a Carboidratos (CBMs) derivados de Metagenômica
• Beneficiário: Bruna Medéia de Campos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/58037-9 - Geração de biblioteca para conversão enzimática de biomassa a partir de metagenoma do solo
• Beneficiário: Fábio Márcio Squina
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores
• Processo FAPESP: 14/04105-4 - Caracterização estrutural e funcional de novas celulases com foco na relação entre domínios catalíticos e CBMs
• Beneficiário: Marcelo Vizoná Liberato
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/11469-1 - Desenvolvimento de uma biblioteca de enzimas a partir de metagenoma de solo
• Beneficiário: Thabata Maria Alvarez
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto