Influence of Structural Features on the Cellular Uptake Behavior of Non-Targeted Polyester-Based Nanocarriers

Autor(es)

de Castro, Carlos E. ; Bonvent, Jean-Jacques ; da Silva, Maria C. C. ; Castro, Fabiane L. F. ; Giacomelli, Fernando C.

• Tipo de documento: Artigo Científico
• Fonte: Macromolecular Bioscience; v. 16, n. 11, p. 1643-1652, NOV 2016.
• Citações Web of Science: 3

Resumo

The development of delivery systems efficiently uptaken by cells is of due importance since sites of drug action are generally localized in subcellular compartments. Herein, naked and core-shell polymeric nanoparticles (NPs) have been produced from poly(lactic-co-glycolic acid)-PLGA, poly(ethylene oxide)-b-poly(e-caprolactone)-PEO-b-PCL, and poly(ethylene oxide)-b-poly(lactic acid)-PEO-b-PLA. The nanostructures are characterized and the cellular uptake behavior is evaluated. The data evidence that cellular uptake is enhanced as the length of the hydrophilic PEO-stabilizing shell reduces and that high negative surface charge restricts cellular uptake. Furthermore, NPs of higher degree of hydrophobicity (PEO-b-PCL) are more efficiently internalized as compared to PEO-b-PLA NPs. Accordingly, taking into account our recent published results({[}1]) and the findings of the current investigation, there should be a compromise regarding protein fouling and cellular uptake as resistance to nonspecific protein adsorption and enhanced cellular uptake are respectively directly and inversely related to the length of the PEO-stabilizing shell. (AU)

• Processo FAPESP: 15/24686-4 - Investigação de efeitos de superfície e presença de coroa protéica no processo de internalização celular de sistemas poliméricos nanoestruturados
• Beneficiário: Carlos Eduardo de Castro
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/22983-9 - Produção de sistemas supramoleculares nanoestruturados a partir de DNA e copolímeros em bloco catiônicos com potencial aplicação em terapia genética
• Beneficiário: Fernando Carlos Giacomelli
• Linha de fomento: Auxílio à Pesquisa - Regular