Investigação de efeitos de superfície e presença de coroa proteica no processo de ...
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| Texto completo | |
| Autor(es): |
de Castro, Carlos E.
;
Bonvent, Jean-Jacques
;
da Silva, Maria C. C.
;
Castro, Fabiane L. F.
;
Giacomelli, Fernando C.
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Macromolecular Bioscience; v. 16, n. 11, p. 1643-1652, NOV 2016. |
| Citações Web of Science: | 4 |
| Resumo | |
The development of delivery systems efficiently uptaken by cells is of due importance since sites of drug action are generally localized in subcellular compartments. Herein, naked and core-shell polymeric nanoparticles (NPs) have been produced from poly(lactic-co-glycolic acid)-PLGA, poly(ethylene oxide)-b-poly(e-caprolactone)-PEO-b-PCL, and poly(ethylene oxide)-b-poly(lactic acid)-PEO-b-PLA. The nanostructures are characterized and the cellular uptake behavior is evaluated. The data evidence that cellular uptake is enhanced as the length of the hydrophilic PEO-stabilizing shell reduces and that high negative surface charge restricts cellular uptake. Furthermore, NPs of higher degree of hydrophobicity (PEO-b-PCL) are more efficiently internalized as compared to PEO-b-PLA NPs. Accordingly, taking into account our recent published results({[}1]) and the findings of the current investigation, there should be a compromise regarding protein fouling and cellular uptake as resistance to nonspecific protein adsorption and enhanced cellular uptake are respectively directly and inversely related to the length of the PEO-stabilizing shell. (AU) | |
| Processo FAPESP: | 15/24686-4 - Investigação de efeitos de superfície e presença de coroa proteica no processo de internalização celular de sistemas poliméricos nanoestruturados |
| Beneficiário: | Carlos Eduardo de Castro |
| Linha de fomento: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 14/22983-9 - Produção de sistemas supramoleculares nanoestruturados a partir de DNA e copolímeros em bloco catiônicos com potencial aplicação em terapia genética |
| Beneficiário: | Fernando Carlos Giacomelli |
| Linha de fomento: | Auxílio à Pesquisa - Regular |