Acrylamide mitigation in French fries using native L-asparaginase from Aspergillus oryzae CCT 3940

Autor(es)

Goncalves Dias, Fernanda Furlan ; Bogusz Junior, Stanislau ; Hantao, Leandro Wang ; Augusto, Fabio ; Sato, Helia Harumi

• Tipo de documento: Artigo Científico
• Fonte: LWT-FOOD SCIENCE AND TECHNOLOGY; v. 76, n. B, SI, p. 222-229, MAR 2017.
• Citações Web of Science: 0

Resumo

The Maillard reaction is responsible for color and flavor formation in fried, roasted and baked foods. However, this reaction can also promote the formation of acrylamide, a potential human carcinogen. The application of L-asparaginase represents a promising method for acrylamide mitigation in heated products by the direct conversion of the precursor L-asparagine to L-aspartic acid and ammonia, which does not lead to additional acrylamide formation. Our research group produced and purified an L-asparaginase from native Aspergillus oryzae CCT 3940 with highly potential for acrylamide mitigation. To verify the enzymatic effectiveness, we compared the native L-asparaginase with the commercial recombinant enzyme. For this purpose, a GC-MS/MS method was developed, optimized and validated for the assessment of acrylamide in French fries. Our results indicate a relationship between the L-asparagine content and acrylamide formation in French fries. The acrylamide concentration of the fried potato treated with the L-asparaginase from A. oryzae CCT 3940 and treated with commercial enzyme reduced 72% and 92%, respectively compared to control sample. Moreover, the L-asparaginase from A. oryzae CCT 3940 showed no L-glutaminase activity, while commercial enzyme promoted a decrease in the L-glutamine content (25%). Our results suggest that L-asparaginase from A. oryzae CCT 3940 may be of great value for acrylamide mitigation. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/24046-7 - Produção, caracterização bioquímica e aplicação de L-asparaginase fúngica
• Beneficiário: Hélia Harumi Sato
• Modalidade de apoio: Auxílio à Pesquisa - Regular