Application of aluminum chloride phthalocyanine-loaded solid lipid nanoparticles for photodynamic inactivation of melanoma cells

Autor(es)

Goto, Patricia L. ; Siqueira-Moura, Marigilson P. ; Tedesco, Antonio C.

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Pharmaceutics; v. 518, n. 1-2, p. 228-241, FEB 25 2017.
• Citações Web of Science: 12

Resumo

Cutaneous melanoma is the most aggressive skin cancer and is particularly resistant to current therapeutic approaches. Photodynamic therapy (PDT) is a well-established photoprocess that is employed to treat some cancers, including non-melanoma skin cancer. Aluminum chloride phthalocyanine (CIAIPc) is used as a photosensitizer in PDT; however, its high hydrophobicity hampers its photodynamic activity under physiological conditions. The aim of this study was to produce solid lipid nanoparticles (SLN) containing CIAIPc using the direct emulsification method. CIAIPc-loaded SLNs (CIAIPc/SLNs) were characterized according to their particle size and distribution, zeta potential, morphology, encapsulation efficiency, stability, and phototoxic action in vitro in B16-F10 melanoma cells. CIAIPc/SLN had a mean diameter between 100 and 200 nm, homogeneous size distribution (polydispersity index <0.3), negative zeta potential, and spherical morphology. The encapsulation efficiency was approximately 100%. The lipid crystallinity was investigated using X-ray diffraction and differential scanning calorimetry and indicated that CIAIPc was integrated into the SLN matrix. The CIAIPc/SLN formulations maintained their physicochemical stability without expelling the drug over a 24-month period. Compared to free CIAIPc, CIAIPc/SLN exerted outstanding phototoxicity effects in vitro against melanoma cells. Therefore, our results demonstrated that the CIAIPc/SLN described in the current study has the potential for use in further preclinical and clinical trials in PDT for melanoma treatment. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
• Beneficiário: Antonio Claudio Tedesco
• Linha de fomento: Auxílio à Pesquisa - Temático