Multifunctional hybrid nanoparticles to enhance photodynamic therapy (PDT) and pho...
| Full text | |
| Author(s): |
Goto, Patricia L.
;
Siqueira-Moura, Marigilson P.
;
Tedesco, Antonio C.
Total Authors: 3
|
| Document type: | Journal article |
| Source: | International Journal of Pharmaceutics; v. 518, n. 1-2, p. 228-241, FEB 25 2017. |
| Web of Science Citations: | 12 |
| Abstract | |
Cutaneous melanoma is the most aggressive skin cancer and is particularly resistant to current therapeutic approaches. Photodynamic therapy (PDT) is a well-established photoprocess that is employed to treat some cancers, including non-melanoma skin cancer. Aluminum chloride phthalocyanine (CIAIPc) is used as a photosensitizer in PDT; however, its high hydrophobicity hampers its photodynamic activity under physiological conditions. The aim of this study was to produce solid lipid nanoparticles (SLN) containing CIAIPc using the direct emulsification method. CIAIPc-loaded SLNs (CIAIPc/SLNs) were characterized according to their particle size and distribution, zeta potential, morphology, encapsulation efficiency, stability, and phototoxic action in vitro in B16-F10 melanoma cells. CIAIPc/SLN had a mean diameter between 100 and 200 nm, homogeneous size distribution (polydispersity index <0.3), negative zeta potential, and spherical morphology. The encapsulation efficiency was approximately 100%. The lipid crystallinity was investigated using X-ray diffraction and differential scanning calorimetry and indicated that CIAIPc was integrated into the SLN matrix. The CIAIPc/SLN formulations maintained their physicochemical stability without expelling the drug over a 24-month period. Compared to free CIAIPc, CIAIPc/SLN exerted outstanding phototoxicity effects in vitro against melanoma cells. Therefore, our results demonstrated that the CIAIPc/SLN described in the current study has the potential for use in further preclinical and clinical trials in PDT for melanoma treatment. (C) 2017 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 13/50181-1 - Use of drugs containing nanocarriers with photosensitizers and/or other active compounds applied to cell therapy and treatment of central nervous system disorders |
| Grantee: | Antonio Claudio Tedesco |
| Support type: | Research Projects - Thematic Grants |