Molecular and morphological surface analysis: effect of filling pastes and cleaning agents on root dentin

Autor(es)

Dainezi, Vanessa Benetello ; Iwamoto, Alexsandra Shizue ; Martin, Airton Abrahao ; Silva Soares, Luis Eduardo ; Hosoya, Yumiko ; Pascon, Fernanda Miori ; Puppin-Rontani, Regina Maria

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 25, n. 1, p. 101-111, JAN-FEB 2017.
• Citações Web of Science: 1

Resumo

Abstract The quality of the dentin root is the most important factor for restoration resin sealing and drives the outcome of endodontic treatment. Objective This study evaluated the effect of different filling pastes and cleaning agents on the root dentin of primary teeth using Fourier-transformed Raman spectroscopy (FT-Raman), micro energy-dispersive X-ray fluorescence (µ-EDXRF) and scanning electron microscopic (SEM) analysis. Material and Methods Eighty roots of primary teeth were endodontically prepared and distributed into 4 groups and filled according to the following filling pastes: Control-no filling (CP), Calen®+zinc oxide (CZ), Calcipex II® (CII), Vitapex® (V). After seven days, filling paste groups were distributed to 4 subgroups according to cleaning agents (n=5): Control-no cleaning (C), Ethanol (E), Tergenform® (T), 35% Phosphoric acid (PA). Then, the roots were sectioned and the dentin root sections were internally evaluated by FT-Raman, µ-EDXRF and SEM. Data was submitted to two-way ANOVA and Tukey tests (α=0.05). Results Regarding filling pastes, there was no significant difference in organic content. CP provided the lowest calcium values and, calcium/phosphoric ratio (Ca/P), and the highest phosphoric values. For cleaning agents there was no difference in organic content when compared to the C; however, T showed significantly higher calcium and Ca/P than PA. All groups showed similar results for phosphorus. The dentin smear layer was present after use of the cleaning agents, except PA. Conclusion The filling pastes changed the inorganic content, however they did not change the organic content. Cleaning agents did not alter the inorganic and organic content. PA cleaned and opened dentin tubules. (AU)

• Processo FAPESP: 05/50811-9 - Estudo do colágeno da dentina por técnicas de microanálise
• Beneficiário: Airton Abrahao Martin
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 01/14384-8 - Estudo de células neoplásicas por espectroscopia de infravermelho próximo
• Beneficiário: Airton Abrahao Martin
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/12757-0 - Estudo de diferentes agentes químicos na Limpeza Pós-Obturação com diferentes excipientes em Dentes Decíduos: Análises Molecular, Morfológica e Micro-Mecânica da dentina radicular
• Beneficiário: Regina Maria Puppin Rontani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/10837-0 - Estudo de diferentes agentes químicos na Limpeza Pós-Obturação com diferentes excipientes em Dentes Decíduos: Análises Molecular, Morfológica e Micro-Mecânica da dentina radicular
• Beneficiário: Vanessa Benetello
• Modalidade de apoio: Bolsas no Brasil - Mestrado