New materials for sample preparation techniques in bioanalysis

Autor(es)

Domingues Nazario, Carlos Eduardo ; Fumes, Bruno Henrique ; da Silva, Meire Ribeiro ; Lancas, Fernando Mauro

• Tipo de documento: Artigo de Revisão
• Fonte: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1043, p. 81-95, FEB 1 2017.
• Citações Web of Science: 19

Resumo

The analysis of biological samples is a complex and difficult task owing to two basic and complementary issues: the high complexity of most biological matrices and the need to determine minute quantities of active substances and contaminants in such complex sample. To succeed in this endeavor samples are usually subject to three steps of a comprehensive analytical methodological approach: sample preparation, analytes isolation (usually utilizing a chromatographic technique) and qualitative/quantitative analysis (usually with the aid of mass spectrometric tools). Owing to the complex nature of bio-samples, and the very low concentration of the target analytes to be determined, selective sample preparation techniques is mandatory in order to overcome the difficulties imposed by these two constraints. During the last decade new chemical synthesis approaches has been developed and optimized, such as sol-gel and molecularly imprinting technologies, allowing the preparation of novel materials for sample preparation including graphene and derivatives, magnetic materials, ionic liquids, molecularly imprinted polymers, and much more. In this contribution we will review these novel techniques and materials, as well as their application to the bioanalysis niche. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/22055-9 - Desenvolvimento de sílica superficialmente modificada com líquidos iônicos e seu uso em técnicas de preparo de amostra
• Beneficiário: Meire Ribeiro da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/15462-5 - Emprego do grafeno como material sorvente em técnicas miniaturizadas de preparo de amostra.
• Beneficiário: Bruno Henrique Fumes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/07347-9 - Desenvolvimento de novos materiais e dispositivos para técnicas miniaturizadas de preparo de amostras na área de segurança alimentar
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/12306-2 - Preparação e avaliação de colunas tubulares abertas porosas (PLOT) para separação de sulfonamidas e seus metabólitos acetilados em amostras alimentícias por nano-LC-MS/MS
• Beneficiário: Meire Ribeiro da Silva
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado