Generation of LIF-independent induced pluripotent stem cells from canine fetal fibroblasts

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Goncalves, N. J. N. ; Bressan, F. F. ; Roballo, K. C. S. ; Meirelles, F. V. ; Xavier, P. L. P. ; Fukumasu, H. ; Williams, C. ; Breen, M. ; Koh, S. ; Sper, R. ; Piedrahita, J. ; Ambrosio, C. E.

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 92, p. 75-82, APR 1 2017.
• Citações Web of Science: 10

Resumo

Takahashi and Yamanaka established the first technique in which transcription factors related to pluripotency are incorporated into the genome of somatic cells to enable reprogramming of these cells. The expression of these transcription factors enables a differentiated somatic cell to reverse its phenotype to an embryonic state, generating induced pluripotent stem cells (iPSCs). iPSCs from canine fetal fibroblasts were produced through lentiviral polycistronic human and mouse vectors (hOSKM/mOSKM), aiming to obtain pluripotent stem cells with similar features to embryonic stem cells (ESC) in this animal model. The cell lines obtained in this study were independent of LIF or any other supplemental inhibitors, resistant to enzymatic procedure (TrypLE Express Enzyme), and dependent on bFGF. Clonal lines were obtained from slightly different protocols with maximum reprogramming efficiency of 0.001%. All colonies were positive for alkaline phosphatase, embryoid body formation, and spontaneous differentiation and expressed high levels of endogenous OCT4 and SOX2. Canine iPSCs developed tumors at 120 days post-injection in vivo. Preliminary chromosomal evaluations were performed by FISH hybridization, revealing no chromosomal abnormality. To the best of our knowledge, this report is the first to describe the ability to reprogram canine somatic cells via lentiviral vectors without supplementation and with resistance to enzymatic action, thereby demonstrating the pluripotency of these cell lines. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 15/09575-1 - Edição gênica por CRISPR-Cas9 na correção da distrofia muscular de Duchenne no modelo canino (GRMD) a partir de células de pluripotência induzida
• Beneficiário: Natalia Juliana Nardelli Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/09631-0 - Estabelecimento e cultivo de iPS a partir de fibroblastos caninos
• Beneficiário: Natalia Juliana Nardelli Gonçalves
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/22915-5 - Geração de células tronco pluripotentes caninas através de mecanismos in vivo e in vitro
• Beneficiário: Natalia Juliana Nardelli Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Doutorado