| Texto completo | |
| Autor(es): |
Pelizzon, Cristiane
;
Carvalho, Carolina da Silva
;
Caballero, Susana
;
Galetti Junior, Pedro Manoel
;
Sanches, Alexandra
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CONSERVATION GENETICS RESOURCES; v. 9, n. 1, p. 17-19, MAR 2017. |
| Citações Web of Science: | 2 |
| Resumo | |
The knowledge of the sex ratio of threatened populations or species is pivotal since sub-optimal sex ratios can affect negatively the population growth and resilience. The vulnerable species, Tapirus terrestris, is rarely studied through traditional field methods and non-invasive genetic analyses have been employed using feces as source of DNA. Here, we evaluated two molecular markers for sex identification of tapirs. Amelogenin gene (AMEL) which is shorter in the Y chromosome (-20pb), failed in the determination of the sex of tapirs, even after the design of specific primers for the species. SRY marker, co-amplified with zinc-finger gene (ZF), had successful produced the sex identity for blood and scat samples. From a total of 45 feaces, 29 had the sex identified after three PCRS (64.4 %). In this way, these molecular markers are reliable tools for sex ratio determination using non-invasive samples of natural populations of this tapirs. (AU) | |
| Processo FAPESP: | 13/19377-7 - A efetividade do maior corredor de mata atlântica em manter conectadas populações de mamíferos de grande porte, como a Anta (Tapirus terrestris) |
| Beneficiário: | Pedro Manoel Galetti Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 07/04073-1 - Ligando paisagem a moléculas: análise genética das populações de anta (Tapirus terrestris) na Mata Atlântica de São Paulo |
| Beneficiário: | Alexandra Sanches |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |