Immune recognition of salivary proteins from the cattle tick Rhipicephalus microplus differs according to the genotype of the bovine host

Autor(es)

Garcia, Gustavo Rocha ; Maruyama, Sandra Regina ; Nelson, Kristina T. ; Chaves Ribeiro, Jose Marcos ; Gardinassi, Luiz Gustavo ; Mendes Maia, Antonio Augusto ; Ferreira, Beatriz Rossetti ; Kooyman, Frans N. J. ; de Miranda Santos, Isabel K. F.

• Tipo de documento: Artigo Científico
• Fonte: PARASITES & VECTORS; v. 10, MAR 14 2017.
• Citações Web of Science: 2

Resumo

Background: Males of the cattle tick Rhipicephalus microplus produce salivary immunoglobulin-binding proteins and allotypic variations in IgG are associated with tick loads in bovines. These findings indicate that antibody responses may be essential to control tick infestations. Infestation loads with cattle ticks are heritable: some breeds carry high loads of reproductively successful ticks, in others, few ticks feed and they reproduce inefficiently. Different patterns of humoral immunity against tick salivary proteins may explain these phenotypes. Methods: We describe the profiles of humoral responses against tick salivary proteins elicited during repeated artificial infestations of bovines of a tick-resistant (Nelore) and a tick-susceptible (Holstein) breed. We measured serum levels of total IgG1, IgG2 and IgE immunoglobulins and of IgG1 and IgG2 antibodies specific for tick salivary proteins. With liquid chromatography followed by mass spectrometry we identified tick salivary proteins that were differentially recognized by serum antibodies from tick-resistant and tick-susceptible bovines in immunoblots of tick salivary proteins separated by two-dimensional electrophoresis. Results: Baseline levels of total IgG1 and IgG2 were significantly higher in tick-susceptible Holsteins compared with resistant Nelores. Significant increases in levels of total IgG1, but not of IgG2 accompanied successive infestations in both breeds. Resistant Nelores presented with significantly higher levels of salivary-specific antibodies before and at the first challenge with tick larvae; however, by the third challenge, tick-susceptible Holsteins presented with significantly higher levels of IgG1 and IgG2 tick salivary protein-specific antibodies. Importantly, sera from tick-resistant Nelores reacted with 39 tick salivary proteins in immunoblots of salivary proteins separated in two dimensions by electrophoresis versus only 21 spots reacting with sera from tick-susceptible Holsteins. Conclusions: Levels of tick saliva-specific antibodies were not directly correlated with infestation phenotypes. However, in spite of receiving apparently lower amounts of tick saliva, tick-resistant bovines recognized more tick salivary proteins. These reactive salivary proteins are putatively involved in several functions of parasitism and blood-feeding. Our results indicate that neutralization by host antibodies of tick salivary proteins involved in parasitism is essential to control tick infestations. (AU)

• Processo FAPESP: 07/51532-1 - Identificacao de antigenos do carrapato rhipicephalus microplus por soros de bovinos resistentes e suscetiveis.
• Beneficiário: Gustavo Rocha Garcia
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/00382-0 - Prospecção do salivoma de carrapatos para descoberta de novos componentes antimicrobianos
• Beneficiário: Gustavo Rocha Garcia
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/15464-0 - RNA-seq de leucócitos de sangue periférico de bovinos: análise funcional do transcriptoma obtido frente à imunização com antígenos recombinantes do carrapato Rhipicephalus microplus e à infestação
• Beneficiário: Sandra Regina Costa Maruyama
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 07/59357-4 - Genes de glandulas salivares do carrapato do boi, rhipicephalus microplus, afetados pela resposta imune do hospedeiro: clonagem e expressao para avaliacao do potencial como antigeno vacinal.
• Beneficiário: Sandra Regina Costa Maruyama
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/51212-2 - Estudo do proteoma e imunoproteoma salivar do carrapato de bovinos, rhipicephalus (boophilus) microplus, para identificacao e caracterizacao de antigenos silenciosos
• Beneficiário: Gustavo Rocha Garcia
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/54041-6 - Estudo dos transcriptomas de glândulas salivares do carrapato do boi, Rhipicephalus (Boophilus) microplus, alimentados em bovinos resistentes e suscetíveis para obtenção de catálogo de genes afetados pela resposta imune do hospedeiro
• Beneficiário: Sandra Regina Costa Maruyama
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/53645-3 - Desenvolvimento de vacina anti-carrapatos: estudo do proteoma e imunoproteoma salivar do carrapato dos bovinos, Rhipicephalus, para identificação e caracterização de antígenos
• Beneficiário: Isabel Kinney Ferreira de Miranda Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/04087-0 - RNA-seq de leucócitos de sangue periférico de bovinos: análise funcional do transcriptoma obtido frente à imunização com antígenos recombinantes do carrapato Rhipicephalus microplus e à infestação
• Beneficiário: Sandra Regina Costa Maruyama
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado