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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Low-level laser therapy (LLLT) accelerates the sternomastoid muscle regeneration process after myonecrosis due to bupivacaine

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Alessi Pissulin, Cristiane Neves ; Henrique Fernandes, Ana Angelica ; Sanchez Orellana, Alejandro Manuel ; Rossi e Silva, Renata Calciolari ; Michelin Matheus, Selma Maria
Número total de Autores: 5
Tipo de documento: Artigo Científico
Citações Web of Science: 8

Background: Because of its long-lasting analgesic action, bupivacaine is an anesthetic used for peripheral nerve block and relief of postoperative pain. Muscle degeneration and neurotoxicity are its main limitations. There is strong evidence that low-level laser therapy (LLLT) assists in muscle and nerve repair. The authors evaluated the effects of a Gallium Arsenide laser (GaAs), on the regeneration of muscle fibers of the sternomastoid muscle and accessory nerve after injection of bupivacaine. Methods: In total, 30 Wistar adult rats were divided into 2 groups: control group (C: n = 15) and laser group (L: n = 15). The groups were subdivided by antimere, with 0.5% bupivacaine injected on the right and 0.9% sodium chloride on the left. LLLT (GaAs 904 nm, 0,05 W, 2.8 J per point) was administered for 5 consecutive days, starting 24 h after injection of the solutions. Seven days after the trial period, blood samples were collectedfor determination of creatine kinase (CK). The sternomastoid nerve was removed for morphological and morphometric analyses; the surface portion of the sternomastoid muscle was used for histopathological and ultrastructural analyses. Muscle CK and TNF alpha. protein levels were measured. Results: The anesthetic promoted myonecrosis and increased muscle CK without neurotoxic effects. The LLLT reduced myonecrosis, characterized by a decrease in muscle CK levels, inflammation, necrosis, and atrophy, as well as the number of central nuclei in the muscle fibers and the percentage of collagen. TNEa values remained constant. Conclusions: LLLT, at the dose used, reduced fibrosis and myonecrosis in the sternomastoid muscle triggered by bupivacaine, accelerating the muscle regeneration process. (C) 2017 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/26649-3 - Avaliação da miotoxicidade e neurotoxicidade (com foco na junção neuromuscular) após aplicação da bupivacaína seguida de laser terapia
Beneficiário:Selma Maria Michelin Matheus
Linha de fomento: Auxílio à Pesquisa - Regular