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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Low-level laser therapy (LLLT) accelerates the sternomastoid muscle regeneration process after myonecrosis due to bupivacaine

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Alessi Pissulin, Cristiane Neves ; Henrique Fernandes, Ana Angelica ; Sanchez Orellana, Alejandro Manuel ; Rossi e Silva, Renata Calciolari ; Michelin Matheus, Selma Maria
Total Authors: 5
Document type: Journal article
Web of Science Citations: 8

Background: Because of its long-lasting analgesic action, bupivacaine is an anesthetic used for peripheral nerve block and relief of postoperative pain. Muscle degeneration and neurotoxicity are its main limitations. There is strong evidence that low-level laser therapy (LLLT) assists in muscle and nerve repair. The authors evaluated the effects of a Gallium Arsenide laser (GaAs), on the regeneration of muscle fibers of the sternomastoid muscle and accessory nerve after injection of bupivacaine. Methods: In total, 30 Wistar adult rats were divided into 2 groups: control group (C: n = 15) and laser group (L: n = 15). The groups were subdivided by antimere, with 0.5% bupivacaine injected on the right and 0.9% sodium chloride on the left. LLLT (GaAs 904 nm, 0,05 W, 2.8 J per point) was administered for 5 consecutive days, starting 24 h after injection of the solutions. Seven days after the trial period, blood samples were collectedfor determination of creatine kinase (CK). The sternomastoid nerve was removed for morphological and morphometric analyses; the surface portion of the sternomastoid muscle was used for histopathological and ultrastructural analyses. Muscle CK and TNF alpha. protein levels were measured. Results: The anesthetic promoted myonecrosis and increased muscle CK without neurotoxic effects. The LLLT reduced myonecrosis, characterized by a decrease in muscle CK levels, inflammation, necrosis, and atrophy, as well as the number of central nuclei in the muscle fibers and the percentage of collagen. TNEa values remained constant. Conclusions: LLLT, at the dose used, reduced fibrosis and myonecrosis in the sternomastoid muscle triggered by bupivacaine, accelerating the muscle regeneration process. (C) 2017 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 13/26649-3 - Evaluation of myotoxicity and neurotoxicity ( neuromuscular junction focus ) after bupivacaine application followed by laser therapy
Grantee:Selma Maria Michelin Matheus
Support type: Regular Research Grants