Integrated microfluidic devices for the synthesis of nanoscale liposomes and lipoplexes

Autor(es)

Balbino, Tiago A. ; Serafin, Juliana M. ; Radaic, Allan ; de Jesus, Marcelo B. ; de la Torre, Lucimara G.

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 152, p. 406-413, APR 1 2017.
• Citações Web of Science: 10

Resumo

In this work, pDNA/cationic liposome (CL) lipoplexes for gene delivery were prepared in one-step using multiple hydrodynamic flow-focusing regions. The microfluidic platform was designed with two distinct regions for the synthesis of liposomes and the subsequent assembly with pDNA, forming lipoplexes. The obtained lipoplexes exhibited appropriate physicochemical characteristics for gene therapy applications under varying conditions of flow rate-ratio (FRR), total volumetric flow rate (QT) and pDNA content (molar charge ratio, R). The CLs were able to condense and retain the pDNA in the vesicular structures with sizes ranging from 140 nm to 250 nm. In vitro transfection assays showed that the lipoplexes prepared in one step by the two-stage configuration achieved similar efficiencies as lipoplexes prepared by conventional bulk processes, in which each step comprises a series of manual operations. The integrated microfluidic platform generates lipoplexes with liposome formation combined in-line with lipoplex assembly, significantly reducing the number of steps usually required to form gene carrier systems. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/14925-6 - Sistemas microfluídicos de focalização hidrodinâmica para a produção de lipossomas catiônicos
• Beneficiário: Tiago Albertini Balbino
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 13/05868-9 - Sistemas microfluídicos para a produção de nanopartículas multifuncionais destinadas à terapia gênica
• Beneficiário: Lucimara Gaziola de la Torre
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/23143-9 - Sistemas microfluídicos para a produção de nanopartículas multifuncionais destinadas à terapia e vacinação gênica
• Beneficiário: Tiago Albertini Balbino
• Modalidade de apoio: Bolsas no Brasil - Doutorado