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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Integrated microfluidic devices for the synthesis of nanoscale liposomes and lipoplexes

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Author(s):
Balbino, Tiago A. ; Serafin, Juliana M. ; Radaic, Allan ; de Jesus, Marcelo B. ; de la Torre, Lucimara G.
Total Authors: 5
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 152, p. 406-413, APR 1 2017.
Web of Science Citations: 10
Abstract

In this work, pDNA/cationic liposome (CL) lipoplexes for gene delivery were prepared in one-step using multiple hydrodynamic flow-focusing regions. The microfluidic platform was designed with two distinct regions for the synthesis of liposomes and the subsequent assembly with pDNA, forming lipoplexes. The obtained lipoplexes exhibited appropriate physicochemical characteristics for gene therapy applications under varying conditions of flow rate-ratio (FRR), total volumetric flow rate (QT) and pDNA content (molar charge ratio, R). The CLs were able to condense and retain the pDNA in the vesicular structures with sizes ranging from 140 nm to 250 nm. In vitro transfection assays showed that the lipoplexes prepared in one step by the two-stage configuration achieved similar efficiencies as lipoplexes prepared by conventional bulk processes, in which each step comprises a series of manual operations. The integrated microfluidic platform generates lipoplexes with liposome formation combined in-line with lipoplex assembly, significantly reducing the number of steps usually required to form gene carrier systems. (C) 2017 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 13/14925-6 - Hydrodynamic Flow-Focusing microfluidic systems for cationic liposome production
Grantee:Tiago Albertini Balbino
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 13/05868-9 - Microfluidic multifunctional nanoparticle production for gene therapy
Grantee:Lucimara Gaziola de la Torre
Support type: Regular Research Grants
FAPESP's process: 12/23143-9 - Microfluidic systems for production of multifunctional nanoparticles for gene and vaccine therapy
Grantee:Tiago Albertini Balbino
Support type: Scholarships in Brazil - Doctorate