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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Alternative Animal and Non-Animal Models for Drug Discovery and Development: Bonus or Burden?

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Autor(es):
Freires, Irlan Almeida ; de Cassia Orlandi Sardi, Janaina ; de Castro, Ricardo Dias ; Rosalen, Pedro Luiz
Número total de Autores: 4
Tipo de documento: Artigo Científico
Fonte: PHARMACEUTICAL RESEARCH; v. 34, n. 4, p. 681-686, APR 2017.
Citações Web of Science: 25
Resumo

Mammalian models have served as a basis for R\&D over the past decades. Nevertheless, these models are expensive, laborious, may yield results that cannot always be translated into the human in vivo situation and, more recently, have reverberated great social and ethical dilemmas. Hence, the prospect of changes in the global scientific scenario and the Three Rs principle (Reduction, Replacement and Refinement) have encouraged the development of alternative methods to the use of mammals. Despite the efforts, suitable alternative tests are not available in all areas of biomedical research, as regulatory acceptance requires time, prior validation and robust financial and scientific investment. In this perspective, we aim to shed light on the concepts, challenges and perspectives for implementation of innovative alternative animal and non-animal methods in scientific research. The applicability and meaningfulness of invertebrate animal models, in silico analysis and reverse pharmacology are discussed, among other aspects of relevance in today's scenario. Overall, the use of alternative models, including Artemia salina (brine shrimp), Caenorhabditis elegans (roundworm), Danio rerio (zebra fish), Drosophila melanogaster (fruit fly), Galleria mellonella (greater waxmoth) and in silico modelling, increased 909% from 1990 to 2015, as compared to 154% of conventional mammals in the same period. Thus, technological and scientific advancements in the fields of toxicology and drug development seem to have diminished the need for mammalian models. Today, however, mammals still remain critically indispensable to provide - in most cases -reliable data subsidizing and validating translation into the clinical setting. (AU)

Processo FAPESP: 11/15984-0 - Avaliação da atividade anti-Candida, antiproliferativa e citotóxica do óleo essencial e frações de Coriandrum sativum L.
Beneficiário:Irlan de Almeida Freires
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 13/25080-7 - Prevalência de cepas de Streptococcus mutans expressando Cnm de crianças e adultos e o papel desta glicoproteína nas interações patógeno-hospedeiro
Beneficiário:Irlan de Almeida Freires
Linha de fomento: Bolsas no Brasil - Doutorado