Evaluation of superficially porous and fully porous columns for analysis of drugs in plasma samples by UHPLC-MS/MS

Autor(es)

Acquaro Junior, Vinicius Ricardo ; Lancas, Fernando Mauro ; Costa Queiroz, Maria Eugenia

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1048, p. 1-9, MAR 24 2017.
• Citações Web of Science: 5

Resumo

This work compares the performance of recently introduced C-18 superficially porous columns (four columns) and C18 fully porous colurims (two columns), with different particle sizes, during the analysis of drugs in plasma samples by MS/MS detection. The following chromatographic parameters were evaluated for all the columns: reduced plate height (h) vs reduced linear velocity (upsilon), impedance vs upsilon, chromatographic run time vs flow rate (mLmin(-1)), backpressure vs flow rate (mLmin(-1)), resolution, peak capacity, asymmetry, and retention factor. By using DAD detection, hydrophobicity, silanol activity, and metal impurities were also assessed for the columns. The columns with charged surface displayed improved chromatographic efficiency for the drugs in their ionized form. The columns with particles smaller than 2 mu m (Cortecs 1.611 mu m, Acquity 1.7 mu m, and Kinetex 1.7 mu m) presented higher chromatographic efficiency for the drugs, which were in their partially ionized form. The generated mathematical models were able to predict the backpressure and chromatographic run time at different flow rates for all the columns. Considering the efficiency, impedance, resolution, peak capacity, retention factor, and hydrophobicity, Cortecs 1.6 pm and Acquity 1.7 mu m were the columns that given the best perforMance during analysis of the target drugs in plasma samples.(c) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/22140-1 - Cromatografia Líquida de Ultra Eficiência Bidimensional com Detecção por Espectrometria de Massas em Tandem (2D-UPLC-MS/MS) para determinação de fármacos em amostras de plasma de pacientes esquizofrênicos
• Beneficiário: Vinicius Ricardo Acquaro Junior
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/07619-1 - Cromatografia líquida acoplada à espectrometria de massas em tandem no modo column switching para análises de endocanabinóides e de fármacos em amostras biológicas
• Beneficiário: Maria Eugênia Queiroz Nassur
• Modalidade de apoio: Auxílio à Pesquisa - Regular