Functional Upregulation of STIM-1/Orai-1-Mediated Store-Operated Ca2+ Contributing to the Hypertension Development Elicited by Chronic EtOH Consumption

Autor(es)

Souza Bomfim, Guilherme Henrique ; Mendez-Lopez, Iago ; Alberto Arranz-Tagarro, Juan ; Ferraz Carbonel, Adriana Aparecida ; Roman-Campos, Danilo ; Fernando Padin, Juan ; Garcia Garcia, Antonio ; Jurkiewicz, Aron ; Jurkiewicz, Neide Hyppolito

• Tipo de documento: Artigo Científico
• Fonte: CURRENT VASCULAR PHARMACOLOGY; v. 15, n. 3, p. 265-281, 2017.
• Citações Web of Science: 3

Resumo

Background: Chronic ethanol (EtOH) consumption has been associated with deleterious effects on the cardiovascular system by abnormal calcium (Ca2+) handling. Store-operated Ca2+ entry (SOCE) is related to cardiovascular remodeling which leads to the hypertension development, and the coupling between STIM-1 (ER Ca2+ sensor) and Orai-1 (channel pore) is a key mechanism to control SOCE through of store-operated Ca2+ channels (SOCCs). However, the role of STIM-1/Orai-1-mediated SOCE and its cross-talk with EtOH-triggered vascular remodeling and hypertension remain poorly understood. We address this subject in the present study by evaluating how chronic EtOH consumption induces alterations in Ca2+ handling via SOCE. Methods: Male Wistar Kyoto (WKY) and Spontaneously Hypertensive (SHR) rats were subjected to the intake of increasing EtOH concentrations (5-20%, for 30 days). Systolic blood pressure (SBP) and EtOH concentration were measured; cardiovascular remodeling was assessed by histomorphometry; and function/expression of STIM-1/Orai-1-mediated Ca2+ influx were evaluated by isometric contraction and western blot experiments. Results: Compared to the WKY-Control, our results show that: (1) chronic EtOH consumption caused a significant elevation of SBP in both strains; (2) cardiac hypertrophy and hypertrophic aortic wall remodeling much more pronounced in WKY-EtOH; (3) decreased capacity of ER to store and release Ca2+; (4) increased STIM-1/Orai-1-mediated SOCCs activation, which was selectively inhibited by YM-58483; and (5) increased expression of STIM-1 in WKY-EtOH and SHR-Control rats. Conclusion: These findings suggest that hypertrophic aortic remodeling and abnormal contraction triggered mainly by Ca2+ overload via STIM-1/Orai-1-mediated SOCE through SOCCs are involved hypertension developed by EtOH consumption. (AU)

• Processo FAPESP: 14/01569-0 - Interferência de drogas produzindo perturbações na interação droga-receptor na hipertensão, na hiperglicemia e na neurotransmissão com ênfase no papel modulador da histamina
• Beneficiário: Neide Hyppolito Jurkiewicz
• Modalidade de apoio: Auxílio à Pesquisa - Regular