Distribution of versican and hyaluronan in the mouse uterus during decidualization

Autor(es)

San Martin, S. ^[1] ; Soto-Suazo, M. ; Zorn, T. M.T

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo (USP). Instituto de Ciências Biomédicas. Departamento de Biologia Celular e do Desenvolvimento - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 36, n. 8, p. 1067-1071, Aug. 2003.
• Área do conhecimento: Ciências Biológicas - Morfologia
• Assunto(s): Histologia Imuno-histoquímica Matriz extracelular Endométrio Implantação do embrião

Resumo

Preparation for embryo implantation requires extensive adaptation of the uterine microenvironment. This process consists of cell proliferation and cell differentiation resulting in the transformation of endometrial fibroblasts into a new type of cell called decidual cell. In the present study, we followed the space-time distribution of versican and hyaluronan (HA) in different tissues of the uterus before and after embryo implantation. Fragments of mouse uteri obtained on the fourth, fifth, sixth and seventh days of pregnancy were fixed in Methacarn, embedded in Paraplast and cut into 5-µm thick sections. HA was detected using a biotinylated fragment of the proteoglycan aggrecan, which binds to this glycosaminoglycan with high affinity and specificity. Versican was detected by a polyclonal antibody. Both reactions were developed by peroxidase methods. Before embryo implantation, both HA and versican were present in the endometrial stroma. However, after embryo implantation, HA disappeared from the decidual region immediately surrounding the implantation chamber, whereas versican accumulated in the same region. The differences observed in the expression of HA and versican suggest that both molecules may participate in the process of endometrial decidualization and/or embryo implantation. (AU)

• Processo FAPESP: 00/04078-4 - Caracterizacao e distribuicao de proteoglicanos da matriz extracelular no processo de decidualizacao do endometrio em camundongos.
• Beneficiário: Sebastian Andres San Martin Henriquez
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 01/12283-0 - Expressão, caracterização e distribuição de alguns tipos de proteoglicanos no endométrio durante a gestação em camundongos: efeito da castração e da reposição hormonal
• Beneficiário: Sebastian Andres San Martin Henriquez
• Linha de fomento: Bolsas no Brasil - Doutorado Direto