| Texto completo | |
| Autor(es): Mostrar menos - |
Martins de Almeida, Breno Fernando
;
Oliveira Silva, Kathlenn Liezbeth
;
Chiku, Vanessa Marim
;
Correa Leal, Aline Aparecida
;
Venturin, Gabriela Lovizutto
;
Narciso, Luis Gustavo
;
Cereijido Bersni Fink, Maria Fernanda
;
Eugenio, Flavia de Rezende
;
Patto dos Santos, Paulo Sergio
;
Ciarlini, Paulo Cesar
;
Felix de Lima, Valeria Marcal
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Immunobiology; v. 222, n. 5, p. 693-703, MAY 2017. |
| Citações Web of Science: | 6 |
| Resumo | |
Canine visceral leishmaniasis (CVL) is known to affect the cellular immunity of infected dogs, through impairing lymphoproliferation and microbicidal mechanisms. This study examined heme oxygenase-1 (HO-1) and its metabolites, oxidative stress and IL-10 levels in CVL and investigated correlations between these parameters. Additionally, the effects of HO-1 inhibition on the lymphoproliferative response and cytokine production in lymph node cells (LNCs) from infected dogs were evaluated. Forty-four dogs, 24 controls and 20 dogs with CVL were selected. Plasma and splenic levels of HO-1, haptoglobin, soluble CD163 receptor, ferritin and IL-10 were determined using capture ELISA. The HO-1 levels and relative gene expression in peripheral blood and bone marrow mononuclear cells were also determined. LNCs proliferation was evaluated with an HO-1 activator and with an HO-1 inhibitor, in the presence of the Leishmania infantum soluble antigen (SAgL), using flow cytometry. HO-1, IL-2, IFN-gamma and IL-10 were also determined in these cultures using capture ELISA. Infected dogs presented oxidative stress and increased HO-1 levels and relative gene expression, with correlation between oxidative stress and HO-1. The substances from heme metabolism and IL-10 were also elevated in the plasma and spleens of infected dogs. IL-10 and HO-1 levels were positively correlated with one another. Inhibition of HO-1 increased LNCs proliferation and decreased IL-10 and IL-2 production in the presence of SAgL. The increased HO-1 metabolism observed in CVL is probably associated with oxidative stress and increased IL-10, which could be one of the mechanisms responsible for inhibition of the lymphoproliferative response in sick dogs. (C) 2016 Elsevier GmbH. All rights reserved. (AU) | |
| Processo FAPESP: | 13/06068-6 - O papel da heme oxigenase-1 na leishmaniose visceral canina |
| Beneficiário: | Breno Fernando Martins de Almeida |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 13/07496-1 - Papel da heme oxigenase 1 na leishmaniose visceral canina |
| Beneficiário: | Valéria Marçal Felix de Lima |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |