| Full text | |
| Author(s): Show less - |
Martins de Almeida, Breno Fernando
;
Oliveira Silva, Kathlenn Liezbeth
;
Chiku, Vanessa Marim
;
Correa Leal, Aline Aparecida
;
Venturin, Gabriela Lovizutto
;
Narciso, Luis Gustavo
;
Cereijido Bersni Fink, Maria Fernanda
;
Eugenio, Flavia de Rezende
;
Patto dos Santos, Paulo Sergio
;
Ciarlini, Paulo Cesar
;
Felix de Lima, Valeria Marcal
Total Authors: 11
|
| Document type: | Journal article |
| Source: | Immunobiology; v. 222, n. 5, p. 693-703, MAY 2017. |
| Web of Science Citations: | 6 |
| Abstract | |
Canine visceral leishmaniasis (CVL) is known to affect the cellular immunity of infected dogs, through impairing lymphoproliferation and microbicidal mechanisms. This study examined heme oxygenase-1 (HO-1) and its metabolites, oxidative stress and IL-10 levels in CVL and investigated correlations between these parameters. Additionally, the effects of HO-1 inhibition on the lymphoproliferative response and cytokine production in lymph node cells (LNCs) from infected dogs were evaluated. Forty-four dogs, 24 controls and 20 dogs with CVL were selected. Plasma and splenic levels of HO-1, haptoglobin, soluble CD163 receptor, ferritin and IL-10 were determined using capture ELISA. The HO-1 levels and relative gene expression in peripheral blood and bone marrow mononuclear cells were also determined. LNCs proliferation was evaluated with an HO-1 activator and with an HO-1 inhibitor, in the presence of the Leishmania infantum soluble antigen (SAgL), using flow cytometry. HO-1, IL-2, IFN-gamma and IL-10 were also determined in these cultures using capture ELISA. Infected dogs presented oxidative stress and increased HO-1 levels and relative gene expression, with correlation between oxidative stress and HO-1. The substances from heme metabolism and IL-10 were also elevated in the plasma and spleens of infected dogs. IL-10 and HO-1 levels were positively correlated with one another. Inhibition of HO-1 increased LNCs proliferation and decreased IL-10 and IL-2 production in the presence of SAgL. The increased HO-1 metabolism observed in CVL is probably associated with oxidative stress and increased IL-10, which could be one of the mechanisms responsible for inhibition of the lymphoproliferative response in sick dogs. (C) 2016 Elsevier GmbH. All rights reserved. (AU) | |
| FAPESP's process: | 13/06068-6 - The role of heme oxygenase-1 on canine visceral leishmaniasis |
| Grantee: | Breno Fernando Martins de Almeida |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/07496-1 - Role of heme oxygenase 1 in canine visceral leishmaniasis |
| Grantee: | Valéria Marçal Felix de Lima |
| Support Opportunities: | Regular Research Grants |