Determination of serum aflatoxin B-1-lysine to evaluate the efficacy of an aflatoxin-adsorbing feed additive in pigs fed an aflatoxin B-1-contaminated diet

Autor(es)

Di Gregorio, Mayra Carraro ; Jager, Alessandra Vincenzi ; Maggio Castro Souto, Pollyana Cristina ; Costa, Aline Alves ; Rottinghaus, George Edwin ; Passarelli, Danielle ; Lemos Budino, Fabio Enrique ; Corassin, Carlos Humberto ; Fernandes Oliveira, Carlos Augusto

• Tipo de documento: Artigo Científico
• Fonte: MYCOTOXIN RESEARCH; v. 33, n. 2, p. 93-102, MAY 2017.
• Citações Web of Science: 2

Resumo

In this study, serum aflatoxin B-1 (AFB(1))-lysine was determined in order to evaluate the in vivo efficacy of a hydrated sodium calcium aluminosilicate (HSCAS) in pigs fed AFB(1). Twenty-four 49-day-old crossbred barrows were maintained in individual cages and allowed ad libitum access to feed and water. A completely randomized design was used with six animals assigned to each of four dietary treatments for 21 days as follows: (A) basal diet (BD), (B) BD supplemented with 0.5 % HSCAS, (C) BD supplemented with 1.1 mg/kg AFB(1), and (D) BD supplemented with 0.5 % HSCAS and 1.1 mg/kg AFB(1). HSCAS was able to alleviate the toxic effects of AFB(1) on pigs and reduce (P < 0.05) the levels of serum AFB(1)-lysine. Cumulative reductions of adduct yield values, calculated through the equation {[}(pg AFB(1)-lysine/mg albumin) / (mu g AFB(1)/kg body weight)], were 53.0, 62.8, and 72.1 after 7, 14, and 21 days of oral exposure, respectively. AFB(1)-lysine has potential as an AFB(1)-specific biomarker for diagnostic purposes and for evaluating the efficacy of chemoprotective interventions in pigs. (AU)

• Processo FAPESP: 12/12705-6 - Determinação de biomarcadores de aflatoxina B1 e aplicabilidade na avaliação da eficiência de adsorventes em suínos
• Beneficiário: Mayra Carraro Di Gregorio
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/20895-4 - Avaliação de adutos AFB1-Lisina e AFB1-N7-guanina como biomarcadores de exposição humana e animal às aflatoxinas
• Beneficiário: Carlos Augusto Fernandes de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático