Dissection of the Effects of Quercetin on Mouse Myocardium

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Santos, Michel Santana ; Oliveira, Evaleide Diniz ; Santos-Miranda, Artur ; Cruz, Jader Santos ; Santana Gondim, Antonio Nei ; Rodrigues Menezes-Filho, Jose Evaldo ; Souza, Diego Santos ; Pinho-da-Silva, Leidiane ; Guedes Jesus, Itamar Couto ; Roman-Campos, Danilo ; Guatimosim, Silvia ; Lara, Aline ; Conde-Garcia, Eduardo Antonio ; Lins Vasconcelos, Carla Maria

• Tipo de documento: Artigo Científico
• Fonte: BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY; v. 120, n. 6, p. 550-559, JUN 2017.
• Citações Web of Science: 4

Resumo

Quercetin is a plant flavonoid with several biological activities. This study aimed to describe quercetin effects on contractile and electrophysiological properties of the cardiac muscle as well as on calcium handling. Quercetin elicited positive inotropism that was significantly reduced by propranolol indicating an involvement of the sympathetic nervous system. In cardiomyocytes, 30 M quercetin increased I-Ca,I-L at 0 mV from -0.95 +/- 0.01 A/F to -1.21 +/- 0.08 A/F. The membrane potential at which 50% of the channels are activated (V-0.5) shifted towards more negative potentials from -13.06 +/- 1.52 mV to -19.26 +/- 1.72 mV and did not alter the slope factor. Furthermore, quercetin increased {[}Ca2+](i) transient by 28% when compared to control. Quercetin accelerated {[}Ca2+](i) transient decay time, which could be attributed to SERCA activation. In resting cardiomyocytes, quercetin did not change amplitude or frequency of Ca2+ sparks. In isolated heart, quercetin increased heart rate and decreased PRi, QTc and duration of the QRS complex. Thus, we showed that quercetin activates -adrenoceptors, leading to increased L-type Ca2+ current and cell-wide intracellular Ca2+ transient without visible changes in Ca2+ sparks. (AU)

• Processo FAPESP: 14/09861-1 - Papel da corrente tardia de sódio nas arritmias cardíacas herdadas e adquiridas: da biofísica à procura de novos alvos terapêuticos
• Beneficiário: Danilo Roman Campos
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores