Postradiation Matrix Metalloproteinase-20 Expression and Its Impact on Dental Micromorphology and Radiation-Related Caries

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Gomes-Silva, Wagner ; Prado-Ribeiro, Ana Carolina ; Brandao, Thais Bianca ; Morais-Faria, Karina ; de Castro Junior, Gilberto ; Mak, Milena Perez ; Lopes, Marcio Ajudarte ; Rocha, Marcelo Marques ; Salo, Tuula ; Tjaderhane, Leo ; de Goes, Mario Fernando ; Santos-Silva, Alan Roger

• Tipo de documento: Artigo Científico
• Fonte: Caries Research; v. 51, n. 3, p. 216-224, 2017.
• Citações Web of Science: 4

Resumo

Recent evidence suggests that head-and-neck radiotherapy (HNRT) increases active forms of matrix metalloproteinase-20 (MMP-20) in human tooth crowns, degrading the dentin-enamel junction (DEJ) and leading to enamel delamination, which is a pivotal step in the formation of radiation-related caries (RRC). Additional participation of enzymatic degradation of organic matrix components in caries progression was attributed to MMP-20 in dentin. Therefore, the current study tested the hypothesis that MMP-20 is overexpressed in the DEJ, dentin-pulp complex components, and carious dentin of post-HNRT patients, leading to detectable micromorphological changes to the enamel and dentin. Thirty-six teeth were studied, including 19 post-HNRT specimens and 17 nonirradiated controls. Optical light microscopy was used to investigate the micromorphological components of the DEJ, dentin-pulp complex components, and carious dentin. The samples were divided into 2 subgroups: nondemineralized ground sections (n = 20) and demineralized histological sections (n = 16). In addition, immunohistochemical analysis using the immunoperoxidase technique was conducted to semiquantitatively assess MMP-20 expression in the DEJ, dentin-pulp complex components, and carious dentin. No apparent damage to the DEJ microstructure or other dentin-pulp complex components was observed and no statistically significant differences were detected in MMP-20 expression (p > 0.05) between the irradiated and control groups. This study rejected the hypothesis that MMP-20 is overexpressed in the DEJ, dentin-pulp complex components, and carious dentin of post-HNRT patients, leading to detectable micromorphological changes. Hence, direct effects of radiation may not be regarded as an independent factor to explain aggressive clinical patterns of RRC. (C) 2017 S. Karger AG, Basel (AU)

• Processo FAPESP: 12/06138-1 - Estudo prospectivo da incidência de trismo induzido por radioterapia em pacientes com carcinoma espinocelular de boca
• Beneficiário: Ana Carolina Prado Ribeiro e Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/18402-8 - Avaliação dos efeitos diretos da radiação sobre o esmalte, a dentina e a polpa dental de pacientes com câncer de cabeça e pescoço
• Beneficiário: Alan Roger dos Santos Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/22059-5 - Análise estrutural do esmalte dental de pacientes com câncer de cabeça e pescoço submetidos à radioterapia.
• Beneficiário: Alan Roger dos Santos Silva
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo