RHD and RHCE genotyping by next-generation sequencing is an effective strategy to identify molecular variants within sickle cell disease patients

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Dezan, Marcia R. ; Ribeiro, Ingrid Helena ; Oliveira, Valeria B. ; Vieira, Juliana B. ; Gomes, Francisco C. ; Franco, Lucas A. M. ; Varuzza, Leonardo ; Ribeiro, Roberto ; Chinoca, Karen Ziza ; Levi, Jose Eduardo ; Krieger, Jose Eduardo ; Pereira, Alexandre Costa ; Gualandro, Sandra F. M. ; Rocha, Vanderson G. ; Mendrone-Junior, Alfredo ; Sabino, Ester Cerdeira ; Dinardo, Carla Luana

• Tipo de documento: Artigo Científico
• Fonte: BLOOD CELLS MOLECULES AND DISEASES; v. 65, p. 8-15, JUN 2017.
• Citações Web of Science: 14

Resumo

Background: The complexity of Rh genetic variation among sickle cell disease (SCD) patients is high. Conventional molecular assays cannot identify all genetic variants already described for the RH locus as well as foresee novel alleles. Sequencing RHD and RHCE is indicated to broaden the search for Rh genetic variants. Aims: To standardize the Next Generation Sequencing (NGS) strategy to assertively identify Rh genetic variants among SCD patients with serologic suspicion of Rh variants and evaluate if it can improve the transfusion support. Methods: Thirty-five SCD patients with unexplained Rh antibodies were enrolled. A NGS-based strategy was developed to genotype RHD and RHCE using gene-specific primers. Genotype and serological data were compared. Results: Data obtained from the NGS-based assay were gene-specific. Ten and 25 variant RHD and RHCE alleles were identified, respectively. Among all cases of unexplained Rh antibodies, 62% had been inaccurately classified by serological analysis and, of these, 73.1% were considered as relevant, as were associated with increased risk of hemolytic reactions and shortage of units suitable for transfusion. Conclusion: The NGS assay designed to genotype RH coding regions was effective and accurate in identifying variants. The proposed strategy clarified the Rh phenotype of most patients, improving transfusion support. (AU)

• Processo FAPESP: 14/50250-6 - Padronização de estratégia molecular custo-efetiva para rastreamento de fenótipos eritrocitários e plaquetários raros em doadores de sangue visando a organização de banco de doadores raros no Estado de SP
• Beneficiário: Ester Cerdeira Sabino
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa em Políticas Públicas para o SUS