Cervicovaginal Levels of Human beta-Defensin 1, 2, 3, and 4 of Reproductive-Aged Women With Chlamydia trachomatis Infection

Autor(es)

Noda-Nicolau, Nathalia Mayumi ; Bastos, Larissa Brito ; Bolpetti, Aline Nascimento ; Silva Pinto, Gabriel Vitor ; Marcolino, Larissa Doddi ; Marconi, Camila ; Tafner Ferreira, Carolina Sanita ; Polettini, Jossimara ; Vieira, Eliane Passarelli ; da Silva, Marcia Guimaraes

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF LOWER GENITAL TRACT DISEASE; v. 21, n. 3, p. 189-192, JUL 2017.
• Citações Web of Science: 2

Resumo

Objective: This study included women attending primary health care units in Botucatu, Sao Paulo, Brazil, to assess the cervicovaginal levels of human beta-defensin (hBD) 1, 2, 3, and 4 during Chlamydia trachomatis infection. Patients and Methods: Cervicovaginal samples were collected for Pap testing and assessing the presence of infection by C. trachomatis, human papillomavirus, Neisseria gonorrhoeae, and Trichomonas vaginalis. Vaginal smears were taken to evaluate local microbiota. Human beta-defensin levels were determined using enzyme-linked immunosorbent assay in cervicovaginal fluid samples. Seventy-four women with normal vaginal microbiota and no evidence of infection were included in hBD quantification assays; 37 tested positive for C. trachomatis and 37 were negative. Statistical analysis was performed using Mann-Whitney U test. Results: Women positive for C. trachomatis had significantly lower cervicovaginal hBD-1, hBD-2, and hBD-3 compared with those who tested negative (hBD-1: 0 pg/mL {[}0-2.1] vs 1.6 pg/mL {[}0-2.4], p < .0001; hBD-2: 0 pg/mL {[}0-3.9] vs 0.61 pg/mL {[}0-8.9], p = .0097; and hBD-3: 0 pg/mL {[}0-4.3] vs 0.28 pg/mL {[}0-8.4], p = .0076). Human beta-defensin 4 was not detected. Conclusions: Lower levels of hBD-1, hBD-2, and hBD-3 in cervicovaginal fluid were detected in the presence of C. trachomatis infection. (AU)

• Processo FAPESP: 12/01278-0 - Avaliação da influência da cervicite por Chlamydia trachomatis na persistência da infecção pelo papilomavírus humano (HPV) e no perfil de citocinas produzidas na secreção cervical de mulheres imunocompetentes
• Beneficiário: Márcia Guimarães da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular