International high-risk clones of Klebsiella pneumoniae KPC-2/CC258 and Escherichia coli CTX-M-15/CC10 in urban lake waters

Autor(es)

Nascimento, Tatiane ; Cantamessa, Rodrigo ; Melo, Luana ; Fernandes, Miriam R. ; Fraga, Edmir ; Dropa, Milena ; Sato, Maria I. Z. ; Cerdeira, Louise ; Lincopan, Nilton

• Tipo de documento: Artigo Científico
• Fonte: Science of The Total Environment; v. 598, p. 910-915, NOV 15 2017.
• Citações Web of Science: 15

Resumo

The emergence of high-risk clones of multidrug-resistant (MDR) bacteria in aquatic environments has generated an important public health problem, creating an urgent need to strengthen surveillance. This study reports the occurrence of clinically significant MDR Enterobacteriaceae and non-fermentative bacteria carrying carbapenemases (KPC-2), extended-spectrum beta-lactamases (CTX-M) and plasmid-mediated quinolone resistance (PMQR) genes in urban lakes and reservoirs, in Southeastern Brazil. In this regard, the detection of hospital-associated lineages of KPC-2-producing Klebsiella pneumoniae belonging to the international clonal complex CC258 (STH) and CTX-M-15-producing Escherichia coli belonging to the international CC10 (ST617), in an urban lake, is reported for the first time. Whole genome sequencing (WGS) analysis of KPC-2-producing K. pneumoniae ST11 revealed that bla(KPC-2) gene was carried by an IncN plasmid on a Tn4401b element. This study support that aquatic environments with public access can act as reservoirs of clinically important MDR bacteria, constituting a potential risk to human and animal health. On the other hand, the detection of high-risk clones highlights the extra-hospital spread of clinically significant bacteria into urban aquatic environments. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/23588-0 - Prevalência e diversidade de bactérias gram-negativas multirresistentes em ambientes aquáticos públicos e fontes de água potável no Estado de São Paulo: impacto clínico e ambiental
• Beneficiário: Tatiane Nascimento
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/08593-9 - Pan-resistoma de Klebsiella pneumoniae e Escherichia coli produtoras de beta-lactamases (KPC-2, CTX-M-8, CTX-M-15) endêmicas no Brasil
• Beneficiário: Nilton Erbet Lincopan Huenuman
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/04725-0 - Monitoramento de bactérias Gram-negativas multirresistentes de importância médica (humana e veterinária): impacto clínico/ambiental e desenvolvimento de alternativas terapêuticas e produtos de inovação tecnológica
• Beneficiário: Nilton Erbet Lincopan Huenuman
• Modalidade de apoio: Auxílio à Pesquisa - Regular