Functional metagenomics of oil-impacted mangrove sediments reveals high abundance of hydrolases of biotechnological interest

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Ottoni, Julia Ronzella ; Cabral, Lucelia ; Pereira de Sousa, Sanderson Tarciso ; Lacerda Junior, Gileno Vieira ; Domingos, Daniela Ferreira ; Soares Junior, Fabio Lino ; Pinheiro da Silva, Mylenne Calciolari ; Marcon, Joelma ; Franco Dias, Armando Cavalcante ; de Melo, Itamar Soares ; de Souza, Anete Pereira ; Andreote, Fernando Dini ; de Oliveira, Valeria Maia

• Tipo de documento: Artigo Científico
• Fonte: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY; v. 33, n. 7 JUL 2017.
• Citações Web of Science: 6

Resumo

Mangroves are located in coastal wetlands and are susceptible to the consequences of oil spills, what may threaten the diversity of microorganisms responsible for the nutrient cycling and the consequent ecosystem functioning. Previous reports show that high concentration of oil favors the incidence of epoxide hydrolases and haloalkane dehalogenases in mangroves. This finding has guided the goals of this study in an attempt to broaden the analysis to other hydrolases and thereby verify whether oil contamination interferes with the prevalence of particular hydrolases and their assigned microorganisms. For this, an in-depth survey of the taxonomic and functional microbial diversity recovered in a fosmid library (Library\_Oil Mgv) constructed from oil-impacted Brazilian mangrove sediment was carried out. Fosmid DNA of the whole library was extracted and submitted to Illumina HiSeq sequencing. The resulting Library Oil\_Mgv dataset was further compared with those obtained by direct sequencing of environmental DNA from Brazilian mangroves (from distinct regions and affected by distinct sources of contamination), focusing on hydrolases with potential use in biotechnological processes. The most abundant hydrolases found were proteases, esterases and amylases, with similar occurrence profile in all datasets. The main microbial groups harboring such hydrolaseen-coding genes were distinct in each mangrove, and in the fosmid library these enzymes were mainly assigned to Chloroflexaceae (for amylases), Planctomycetaceae (for esterases) and Bradyrhizobiaceae (for proteases). Assembly and analysis of Library\_Oil Mgv reads revealed three potentially novel enzymes, one epoxide hydrolase, one xylanase and one amylase, to be further investigated via heterologous expression assays. (AU)

• Processo FAPESP: 10/51981-3 - Prospeccao e analise funcional de enzimas provenientes de microbiota de manguezais do estado de sao paulo.
• Beneficiário: Júlia Ronzella Ottoni
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/16850-0 - Análise da diversidade funcional e bioprospecção de genes de degradação de hidrocarbonetos e de transformação de metais pesados em sedimentos de manguezal
• Beneficiário: Lucélia Cabral
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/14534-4 - Metatranscriptômica e contexto genômico de comunidades microbianas envolvidas em ciclos biogeoquímicos em manguezais
• Beneficiário: Armando Cavalcante Franco Dias
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/15519-3 - Prospecção de biossurfactantes a partir de microbiota de manguezais
• Beneficiário: Daniela Ferreira Domingos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/09386-9 - Análise integrada de metagenômica e metatranscriptômica do solo da Caatinga e prospecção de genes envolvidos na degradação de lignina.
• Beneficiário: Gileno Vieira Lacerda Júnior
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/22555-4 - Investigação de genes envolvidos na biodegradação de hidrocarbonetos aromáticos a partir do metagenoma de mangue impactado com petróleo
• Beneficiário: Sanderson Tarciso Pereira de Sousa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/50809-5 - Prospecção de metagenoma microbiano de sedimentos de manguezais na busca por novos compostos bioativos
• Beneficiário: Valeria Maia Merzel
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular