Functional metagenomics of oil-impacted mangrove sediments reveals high abundance of hydrolases of biotechnological interest

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Ottoni, Julia Ronzella ; Cabral, Lucelia ; Pereira de Sousa, Sanderson Tarciso ; Lacerda Junior, Gileno Vieira ; Domingos, Daniela Ferreira ; Soares Junior, Fabio Lino ; Pinheiro da Silva, Mylenne Calciolari ; Marcon, Joelma ; Franco Dias, Armando Cavalcante ; de Melo, Itamar Soares ; de Souza, Anete Pereira ; Andreote, Fernando Dini ; de Oliveira, Valeria Maia

• Document type: Journal article
• Source: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY; v. 33, n. 7 JUL 2017.
• Web of Science Citations: 6

Abstract

Mangroves are located in coastal wetlands and are susceptible to the consequences of oil spills, what may threaten the diversity of microorganisms responsible for the nutrient cycling and the consequent ecosystem functioning. Previous reports show that high concentration of oil favors the incidence of epoxide hydrolases and haloalkane dehalogenases in mangroves. This finding has guided the goals of this study in an attempt to broaden the analysis to other hydrolases and thereby verify whether oil contamination interferes with the prevalence of particular hydrolases and their assigned microorganisms. For this, an in-depth survey of the taxonomic and functional microbial diversity recovered in a fosmid library (Library\_Oil Mgv) constructed from oil-impacted Brazilian mangrove sediment was carried out. Fosmid DNA of the whole library was extracted and submitted to Illumina HiSeq sequencing. The resulting Library Oil\_Mgv dataset was further compared with those obtained by direct sequencing of environmental DNA from Brazilian mangroves (from distinct regions and affected by distinct sources of contamination), focusing on hydrolases with potential use in biotechnological processes. The most abundant hydrolases found were proteases, esterases and amylases, with similar occurrence profile in all datasets. The main microbial groups harboring such hydrolaseen-coding genes were distinct in each mangrove, and in the fosmid library these enzymes were mainly assigned to Chloroflexaceae (for amylases), Planctomycetaceae (for esterases) and Bradyrhizobiaceae (for proteases). Assembly and analysis of Library\_Oil Mgv reads revealed three potentially novel enzymes, one epoxide hydrolase, one xylanase and one amylase, to be further investigated via heterologous expression assays. (AU)

• FAPESP's process: 12/16850-0 - Assessment of the functional diversity and bioprospection of genes involved in degradation of hydrocarbons and transformation of heavy metals in mangrove sediments
• Grantee: Lucélia Cabral
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/14534-4 - METATRANSCRIPTOMICS AND GENOMIC CONTEXT IN MICROBIAL COMMUNITIES INVOLVED IN THE BIOGEOCHEMICAL CYCLES IN MANGROVES
• Grantee: Armando Cavalcante Franco Dias
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 10/15519-3 - Prospecting of biosurfactants from microbiota of mangroves
• Grantee: Daniela Ferreira Domingos
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/09386-9 - Integrated metatranscriptomic and metagenomic analyses of Caatinga soil and genes prospection involved on the liginin degradation.
• Grantee: Gileno Vieira Lacerda Júnior
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/22555-4 - Investigation of genes involved in biodegradation of aromatic hydrocarbons from metagenomic mangrove impacted with petroleum
• Grantee: Sanderson Tarciso Pereira de Sousa
• Support Opportunities: Scholarships in Brazil - Doctorate