Scholarship 13/09386-9 - Bioprospecção, Diversidade microbiana - BV FAPESP
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Integrated metatranscriptomic and metagenomic analyses of Caatinga soil and genes prospection involved on the liginin degradation.

Grant number: 13/09386-9
Support Opportunities:Scholarships in Brazil - Doctorate
Start date until: August 01, 2013
End date until: February 28, 2017
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Valeria Maia Merzel
Grantee:Gileno Vieira Lacerda Júnior
Host Institution: Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA). Universidade Estadual de Campinas (UNICAMP). Paulínia , SP, Brazil

Abstract

The Caatinga is an exclusively Brazilian biome, this mean that its biological patrimony can not be found anywhere else on the planet. Nevertheless, there is few studies done on Caatinga biological information Caatinga, being limited on flora and fauna studies. The biome is considered a region with extreme weather conditions such as high temperatures and solar radiation, high acidity and water deficit and also expected to hold microorganisms adapted to these conditions (extremophiles) that can be explored for the identification of unique and versatile enzyme arsenals (extremozymes) with wide industrial application. The microbial diversity on native Caatinga soil and its biotechnological potential are still poorly explored and existing studies just involve the analysis of cultivable microbiota. As approximately 99% of microorganisms in the soil can not be isolated by conventional methods of cultivation technologies, using molecular analysis of environment genetic material, such as "metagenomics", are needed to provide more precise information on the diversity and potential Bt microbial community. Lignin, the second most abundant biopolymer in the biosphere, is the largest source of aromatic compounds present in the soil. Soil microorganisms are able to decompose lignin using a set of ligninolytics enzymes that can also be used in other processes such as detoxification of industrial wastewater in the paper industry and biofuels, among others. Therefore, it is possible to explore new degradation pathway of these compound in soil microbes involved in the nutrient cycling. This project proposes the first time an integrated metatranscriptomic and metagenomic analyses of Caatinga soil for analyzing the structure of microbial communities and the expression of genes in soil samples in the rainy and dry seasons and identify thermostable ligninolytics enzymes. Nucleic acids (DNA and RNA) of the soil samples should be extracted and cloning for metagenomic library construction. The fosmidial DNA library and total RNA will be subjected to large-scale sequencing using the Roche 454 pyrosequencing platform for further analysis in silic. The clones library will be screened searching for genes involved in lignin degradation due biological assays based on the sequence function and similarity. This study may contribute to a understand microbial responses according to environmental changes in this biome. It is expected to find a microbial community carrying unknown genes with interesting properties involved in lignin degradation.

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DELFORNO, TIAGO PALLADINO; LACERDA JUNIOR, GILENO VIEIRA; NORONHA, MELLINE F.; SAKAMOTO, ISABEL K.; VARESCHE, MARIA BERNADETE A.; OLIVEIRA, VALERIA M.. Microbial diversity of a full-scale UASB reactor applied to poultry slaughterhouse wastewater treatment: integration of 16S rRNA gene amplicon and shotgun metagenomic sequencing. MICROBIOLOGYOPEN, v. 6, n. 3, . (13/09386-9, 14/16426-0)
LACERDA-JUNIOR, V, GILENO; NORONHA, MELLINE F.; CABRAL, LUCELIA; DELFORNO, THAGO P.; PEREIRA DE SOUSA, SANDERSON TARCISO; FERNANDES-JUNIOR, I, PAULO; MELO, TAMER S.; OLIVEIRA, VALERIA M.. Land Use and Seasonal Effects on the Soil Microbiome of a Brazilian Dry Forest. FRONTIERS IN MICROBIOLOGY, v. 10, . (13/09386-9)
LACERDA JUNIOR, GILENO V.; NORONHA, MELLINE F.; DE SOUSA, SANDERSON TARCISO P.; CABRAL, LUCELIA; DOMINGOS, DANIELA F.; SABER, IRIAN L.; DE MELO, ITAMAR S.; OLIVEIRA, VALERIA M.. Potential of semiarid soil from Caatinga biome as a novel source for mining lignocellulose-degrading enzymes. FEMS MICROBIOLOGY ECOLOGY, v. 93, n. 2, . (13/09386-9)
NORONHA, MELLINE FONTES; LACERDA JUNIOR, GILENO VIEIRA; GILBERT, JACK A.; DE OLIVEIRA, VALERIA MAIA. Taxonomic and functional patterns across soil microbial communities of global biomes. Science of The Total Environment, v. 609, p. 1064-1074, . (13/09386-9)
OTTONI, JULIA RONZELLA; CABRAL, LUCELIA; PEREIRA DE SOUSA, SANDERSON TARCISO; LACERDA JUNIOR, GILENO VIEIRA; DOMINGOS, DANIELA FERREIRA; SOARES JUNIOR, FABIO LINO; PINHEIRO DA SILVA, MYLENNE CALCIOLARI; MARCON, JOELMA; FRANCO DIAS, ARMANDO CAVALCANTE; DE MELO, ITAMAR SOARES; et al. Functional metagenomics of oil-impacted mangrove sediments reveals high abundance of hydrolases of biotechnological interest. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 33, n. 7, . (12/16850-0, 12/14534-4, 10/15519-3, 13/09386-9, 13/22555-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LACERDA JÚNIOR, Gileno Vieira. Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation. 2017. 122 f. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia.

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