beta-Lactam resistance and vancomycin heteroresistance in Staphylococcus spp. isolated from bovine subclinical mastitis

Autor(es)

Mello, Priscila Luiza ; Pinheiro, Luiza ; Martins, Lisiane de Almeida ; Vasconcelos Paiva Brito, Maria Aparecida ; Ribeiro de Souza da Cunha, Maria de Lourdes

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF DAIRY SCIENCE; v. 100, n. 8, p. 6567-6571, AUG 2017.
• Citações Web of Science: 2

Resumo

The use of antimicrobial agents has led to the emergence of resistant bacterial strains over a relatively short period. Furthermore, Staphylococcus spp. can produce beta-lactarnase, which explains the survival of these strains in a focus of infection despite the use of a beta-lactam antibiotic. The aim of this study was to evaluate the resistance of Staphylococcus spp. isolated from bovine subclinical mastitis to oxacillin and vancomycin (by minimum inhibitory concentration) and to detect vancomycin heteroresistance by a screening method. We also evaluated beta-lactamase production and resistance due to hyperproduction of this enzyme and investigated the mecA and mecC genes and performed staphylococcal cassette chromosome mec typing. For this purpose, 181 Staphylococcus spp. isolated from mastitis subclinical bovine were analyzed. Using the phenotypic method, 33 (18.2%) of Staphylococcus spp. were resistant to oxacillin. In contrast, all isolates were susceptible to vancomycin, and heteroresistance was detected by the screening method in 13 isolates. Production of beta-lactamase was observed in 174 (96%) of the Staphylococcus spp. isolates. The mecA gene was detected in 8 isolates, all of them belonging to the species Staphylococcus epidermidis, and staphylococcal cassette chromosome mec typing revealed the presence of type I and type IV isolates. (AU)

• Processo FAPESP: 12/24135-0 - Staphylococcus spp. ISOLADOS DE MASTITE SUBCLÍNICA BOVINA EM DIFERENTES ESTADOS BRASILEIROS: RESISTÊNCIA ANTIMICROBIANA, DETECÇÃO DOS FATORES DE VIRULÊNCIA E IDENTIFICAÇÃO DO PERFIL CLONAL.
• Beneficiário: Priscila Luiza Mello
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/01401-4 - Staphylococcus spp. isolados de mastite subclínica bovina em diferentes estados brasileiros: resistência antimicrobiana, detecção dos fatores de virulência e identificação do perfil clonal
• Beneficiário: Maria de Lourdes Ribeiro de Souza da Cunha
• Linha de fomento: Auxílio à Pesquisa - Regular