Identification of karyopherins involved in the nuclear import of RNA exosome subunit Rrp6 in Saccharomyces cerevisiae

Autor(es)

Gonzales-Zubiate, Fernando A. ; Okuda, Ellen K. ; Da Cunha, Julia P. C. ; Oliveira, Carla Columbano

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biological Chemistry; v. 292, n. 29, p. 12267-12284, JUL 21 2017.
• Citações Web of Science: 1

Resumo

The exosome is a conserved multiprotein complex essential for RNA processing and degradation. The nuclear exosome is a key factor for pre-rRNA processing through the activity of its catalytic subunits, Rrp6 and Rrp44. In Saccharomyces cerevisiae, Rrp6 is exclusively nuclear and has been shown to interact with exosome cofactors. With the aim of analyzing proteins associated with the nuclear exosome, in this work, we purified the complex with Rrp6-TAP, identified the co-purified proteins by mass spectrometry, and found karyopherins to be one of the major groups of proteins enriched in the samples. By investigating the biological importance of these protein interactions, we identified Srp1, Kap95, and Sxm1 as the most important karyopherins for Rrp6 nuclear import and the nuclear localization signals recognized by them. Based on the results shown here, we propose a model of multiple pathways for the transport of Rrp6 to the nucleus. (AU)

• Processo FAPESP: 12/50196-6 - Caracterização bioquímica e estrutural das interações do exossomo de levedura com proteínas reguladoras com proteínas reguladoras de sua atividade
• Beneficiário: Fernando Alexis Gonzales Zubiate
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/51842-3 - SMOLBNET2: estudo de estrutura de proteínas componentes e reguladoras do exossomo de Archaea e de levedura
• Beneficiário: Carla Columbano de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/06477-9 - Estudo funcional de componentes do exossomo e do spliceossomo de Saccharomyces cerevisiae e de seu papel no controle pós-transcricional de expressão gênica
• Beneficiário: Carla Columbano de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático