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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of karyopherins involved in the nuclear import of RNA exosome subunit Rrp6 in Saccharomyces cerevisiae

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Author(s):
Gonzales-Zubiate, Fernando A. ; Okuda, Ellen K. ; Da Cunha, Julia P. C. ; Oliveira, Carla Columbano
Total Authors: 4
Document type: Journal article
Source: Journal of Biological Chemistry; v. 292, n. 29, p. 12267-12284, JUL 21 2017.
Web of Science Citations: 1
Abstract

The exosome is a conserved multiprotein complex essential for RNA processing and degradation. The nuclear exosome is a key factor for pre-rRNA processing through the activity of its catalytic subunits, Rrp6 and Rrp44. In Saccharomyces cerevisiae, Rrp6 is exclusively nuclear and has been shown to interact with exosome cofactors. With the aim of analyzing proteins associated with the nuclear exosome, in this work, we purified the complex with Rrp6-TAP, identified the co-purified proteins by mass spectrometry, and found karyopherins to be one of the major groups of proteins enriched in the samples. By investigating the biological importance of these protein interactions, we identified Srp1, Kap95, and Sxm1 as the most important karyopherins for Rrp6 nuclear import and the nuclear localization signals recognized by them. Based on the results shown here, we propose a model of multiple pathways for the transport of Rrp6 to the nucleus. (AU)

FAPESP's process: 12/50196-6 - Biochemical and structural characterization of yeast exosome interactions with regulatory proteins of their activity
Grantee:Fernando Alexis Gonzales Zubiate
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 10/51842-3 - Structural study of protein components of the exosome and some of its regulatory factors from Archaea and yeast
Grantee:Carla Columbano de Oliveira
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/06477-9 - Functional study of the components of the Saccharomyces cerevisiae exosome and the spliceosome, and their role in posttranscriptional control of gene expression
Grantee:Carla Columbano de Oliveira
Support Opportunities: Research Projects - Thematic Grants