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Study of the celular transport mechanism of the exosome's subunits in Saccharomyces cerevisiae

Grant number: 17/17777-9
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): January 01, 2018
Effective date (End): February 02, 2020
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Carla Columbano de Oliveira
Grantee:Ellen Kazumi Okuda
Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:15/06477-9 - Functional study of the components of the Saccharomyces cerevisiae exosome and the spliceosome, and their role in posttranscriptional control of gene expression, AP.TEM
Associated scholarship(s):18/19451-6 - Study of the cellular transport mechanism of the RNA exosome subunit Rrp44 in Saccharomyces cerevisiae, BE.EP.MS


Exosome is a multiprotein complex responsible for processing and degradation of many types of RNAs in eukaryotic cells. This complex localizes both to the nucleus and cytoplasm. The exosome complex has two catalytic subunits: Rrp6, found only in the nucleus, and Rrp44, found in the nucleus and in the cytoplasm of Saccharomyces cerevisiae. The Rrp44 is an essential protein of the RNase II class, which has endonucleolytic and exonucleolytic activities. Due to the importance of the exosome for the posttranscriptional control of gene expression both in the nucleus and in the cytoplasm, our laboratory has started a study on the cellular transport of the exosome subunit Rrp6, which resulted in a recent publication on the identification of the karyopherins involved in the nuclear import of this exosome subunit. Despite this initial information, little is known about the mechanism responsible for the transport of other exosome subunits. To continue that work, nuclear localization signals (NLS) were identified in the Rrp44 subunit sequence, suggesting that this protein may also be transported to the nucleus independently of the exosome complex. This project aims at the identification of the pathways involved in the nuclear transport of Rrp44, as well as of other exosome subunits.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
OKUDA, ELLEN K.; GONZALES-ZUBIATE, FERNANDO A.; GADAL, OLIVIER; OLIVEIRA, CARLA C.. Nucleolar localization of the yeast RNA exosome subunit Rrp44 hints at early pre-rRNA processing as its main function. Journal of Biological Chemistry, v. 295, n. 32, p. 11195-11213, . (17/17777-9, 15/06477-9, 18/19451-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
OKUDA, Ellen Kazumi. Study of the cellular transport mechanism of Exosome complex subunits in Saccharomyces cerevisiae. 2020. Master's Dissertation - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.

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