Vitamin D deficiency aggravates ischemic acute kidney injury in rats

Autor(es)

de Braganca, Ana Carolina ; Volpini, Rildo A. ; Canale, Daniele ; Goncalves, Janaina G. ; Shimizu, Maria Heloisa M. ; Sanches, Talita R. ; Seguro, Antonio C. ; Andrade, Lucia

• Tipo de documento: Artigo Científico
• Fonte: PHYSIOLOGICAL REPORTS; v. 3, n. 3 MAR 2015.
• Citações Web of Science: 14

Resumo

Vitamin D deficiency (VDD) increases the risk of death in hospitalized patients. Renal ischemia/reperfusion injury (IRI) induces acute kidney injury (AKI), which activates cell cycle inhibitors, including p21, a cyclindependent kinase inhibitor and genomic target of 25-hydroxyvitamin D, which is in turn a potent immunomodulator with antiproliferative effects. In this study, we assess the impact of VDD in renal IRI. Wistar rats were divided into groups, each evaluated for 30 days: control (receiving a standard diet); VDD (receiving a vitamin D-free diet); IRI (receiving a standard diet and subjected to 45-min bilateral renal ischemia on day 28); and VDD + IRI (receiving a vitamin D-free diet and subjected to 45-min bilateral renal ischemia on day 28). At 48 h after IRI, animals were euthanized; blood, urine, and kidney tissue samples were collected. Compared with IRI rats, VDD + IRI rats showed a more severe decrease in glomerular filtration rate, greater urinary protein excretion, a higher kidney/body weight ratio and lower renal aquaporin 2 expression, as well as greater morphological damage, characterized by increased interstitial area and tubular necrosis. Our results suggest that the severity of tubular damage in IRI may be associated with downregulation of vitamin D receptors and p21. VDD increases renal inflammation, cell proliferation and cell injury in ischemic AKI. (AU)

• Processo FAPESP: 10/52294-0 - Avaliação do papel da vitamina D na evolução da lesão renal aguda pós-isquêmica
• Beneficiário: Rildo Aparecido Volpini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/03025-1 - Estudo fisiopatológico dos transportadores urinários ao longo do nefron em camundongos nocaute para gene Klotho: Importância da proteína klotho no transporte tubular, no mecanismos de acidificação e na concentração e diluição urinária
• Beneficiário: Talita Rojas Cunha Sanches
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado