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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Vitamin B-12-induced spermatogenesis recovery in cimetidine-treated rats: effect on the spermatogonia number and sperm concentration

Texto completo
Autor(es):
Beltrame, Flavia L. ; Sasso-Cerri, Estela
Número total de Autores: 2
Tipo de documento: Artigo Científico
Fonte: ASIAN JOURNAL OF ANDROLOGY; v. 19, n. 5, p. 567-572, SEP-OCT 2017.
Citações Web of Science: 3
Resumo

The H-2-receptor antagonist cimetidine is an antiulcer drug also used for the treatment of cancer due to its antiangiogenic effect. However, this drug has caused structural changes in the seminiferous tubules. Vitamin B-12 has been used as a therapeutic agent for the treatment of male infertility. The supplementation of rats with vitamin B-12 during cimetidine treatment has recovered the damaged seminiferous tubules, but how this vitamin restores the seminiferous epithelium has not been clarified. In this study, we evaluated whether vitamin B-12 improves the number of spermatogonia, spermatocytes, and sperm concentration in cimetidine-treated rats. Adult male rats were treated for 50 days as follows: cimetidine group received 100 mg kg(-1) b.w. of cimetidine, cimetidine-B-12 group received cimetidine and 3 mu g of vitamin B-12-hydroxocobalamin, B-12 group received only 3 mu g of vitamin, and control group received saline. Sperm concentration was calculated and historesin-embedded testes sections were used for the quantitative analyses of spermatogonia (A; In/B) and spermatocytes. TUNEL method and PCNA immunofluorescence were performed. Cimetidine caused a significant reduction in sperm concentration. TUNEL-positive spermatogonia and spermatocytes were correlated to a significant reduction in the number of these cells. In cimetidine-B-12 group, sperm concentration was higher than cimetidine group and a significant increase in the number of spermatogonia (stages II-VI) was correlated to a high incidence of PCNA-immunolabeled spermatogonia and spermatocytes. The results show that the supplementation of rats with vitamin B-12 during cimetidine treatment increases sperm concentration and exerts a potential effect in the recovery of spermatogonia and spermatocytes. (AU)

Processo FAPESP: 12/23845-3 - Avaliação do efeito da Vitamina B12 sobre as fases mitótica e meiótica da espermatogênese e sobre o DNA espermático de ratos tratados com cimetidina
Beneficiário:Estela Sasso Cerri
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 06/54776-6 - Avaliação morfológica e morfométrica de testículos de ratos tratados com cimetidina, com ênfase na célula de Sertoli e na vascularização intersticial
Beneficiário:Estela Sasso Cerri
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/25322-0 - Efeito da cimetidina na histoarquitetura epididimária e avaliação do DNA espermático de ratos tratados com cimetidina e suplementados com Vitamina B12
Beneficiário:Flávia Luciana Beltrame
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 10/02409-5 - Aspectos histopatológicos do tecido intersticial de ratos tratados com cimetidina e participação da Vitamina B12 na reparação da espermatogênese
Beneficiário:Flávia Luciana Beltrame
Linha de fomento: Bolsas no Brasil - Doutorado