Submucosal neurons and enteric glial cells expressing the P2X7 receptor in rat experimental colitis

Autor(es)

da Silva, Marcos Vinicius ; Marosti, Aline Rosa ; Mendes, Cristina Eusebio ; Palombit, Kelly ; Castelucci, Patricia

• Tipo de documento: Artigo Científico
• Fonte: ACTA HISTOCHEMICA; v. 119, n. 5, p. 481-494, 2017.
• Citações Web of Science: 3

Resumo

The aim of this study was to evaluate the effect of ulcerative colitis on the submucosal neurons and glial cells of the submucosal ganglia of rats. 2,4,6-Trinitrobenzene sulfonic acid (TNBS; colitis group) was administered in the colon to induce ulcerative colitis, and distal colons were collected after 24 h. The colitis rats were compared with those in the sham and control groups. Double labelling of the P2X7 receptor with calbindin (marker for intrinsic primary afferent neurons, IPANs, submucosal plexus), calretinin (marker for secretory and vasodilator neurons of the submucosal plexus), HuC/D and S100 beta was performed in the submucosal plexus. The density (neurons per area) of submucosal neurons positive for the P2X7 receptor, calbindin, calretinin and HuC/D decreased by 21%, 34%, 8.2% and 28%, respectively, in the treated group. In addition, the density of enteric glial cells in the submucosal plexus decreased by 33%. The profile areas of calbindin-immunoreactive neurons decreased by 25%. Histological analysis revealed increased lamina propria and decreased collagen in the colitis group. This study demonstrated that ulcerative colitis affected secretory and vasodilatory neurons, IPANs and enteric glia of the submucosal plexus expressing the P2X7 receptor. (AU)

• Processo FAPESP: 12/00259-1 - Papel do antagonista brilliant blue green (BBG) sobre o receptor P2X7 e o sistema nervoso entérico do íleo de ratos submetidos a isquemia e reperfusão intestinal: estudo morfofuncional
• Beneficiário: Patricia Castelucci
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/05314-5 - Estudo do código químico e da expressão dos receptores P2X2 E P2X7 no sistema nervoso entérico do íleo de ratos submetidos a isquemia/reperfusão intestinal de artérias ileais
• Beneficiário: Patricia Castelucci
• Modalidade de apoio: Auxílio à Pesquisa - Regular