Selective removal of carious lesion with Er:YAG laser followed by dentin biomodification with chitosan

Autor(es)

Curylofo-Zotti, Fabiana A. ; Tanta, Gabriela Solano ; Zucoloto, Miriane Lucindo ; Souza-Gabriel, Aline E. ; Corona, Silmara A. M.

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 32, n. 7, p. 1595-1603, SEP 2017.
• Citações Web of Science: 5

Resumo

The aim of this study was to evaluate the effect of Er:YAG laser for selective removal of carious lesion, followed by biomodification with chitosan gel where the subsurface microhardness, chemical composition, and morphological changes of the residual caries-affected dentin were examined. Artificial dentinal lesions were created by pH-cycling method (14 days) in 104 bovine specimens (5 x 5 mm). Specimens were randomly divided according to the carious removal method: bur (low-speed handpiece) or Er:YAG laser (250 mJ/4 Hz). Specimens were treated with 35% phosphoric acid and were subdivided into two groups according to dentin biomodification: without chitosan (control) and 2.5% chitosan. Forty specimens were restored with an adhesive system and composite resin. Subsurface microhardness tests were performed in sound dentin, caries-affected dentin, residual caries-affected dentin, and after the restoration. The other 64 specimens were subjected to SEM-EDS atomic analysis. Data were statistically analyzed (p < 0.05). After the Er:YAG laser excavation, the microhardness value of residual caries-affected dentin was higher (p < 0.05) than bur-treated dentin. A significant decrease in the amount of Ca, P, and Ca/P ratio was found after the removal of carious lesions with Er:YAG laser (p < 0.05). The biomodification with chitosan did not influence the microhardness and atomic percentage of Ca, P, and Ca/P ratio of residual caries-affected dentin (p > 0.05). SEM analysis showed morphological changes on residual caries-affected dentin (p > 0.05). The selective removal of carious dentin with Er:YAG laser increased microhardness of residual caries-affected dentin, changing its surface morphology and chemical composition. The biomodification with chitosan did not influence the structural and chemical composition of residual caries-affected dentin. (AU)

• Processo FAPESP: 14/23654-9 - Remoção seletiva de lesão de cárie com Laser de Er:YAG e reumidificação da dentina com solução de quitosana
• Beneficiário: Fabiana Almeida Curylofo Zotti
• Modalidade de apoio: Bolsas no Brasil - Doutorado