Participation of extracellular signal-regulated kinases 1/2 in osteoblast and adipocyte differentiation of mesenchymal stem cells grown on titanium surfaces

Autor(es)

Silva, Heitor F. ; Abuna, Rodrigo P. F. ; Lopes, Helena B. ; Francischini, Marcelo S. ; de Oliveira, Paulo T. ; Rosa, Adalberto L. ; Beloti, Marcio M.

• Tipo de documento: Artigo Científico
• Fonte: European Journal of Oral Sciences; v. 125, n. 5, p. 355-360, OCT 2017.
• Citações Web of Science: 4

Resumo

Osteoblasts and adipocytes coexist in the implantation site and affect the process of titanium (Ti) osseointegration. As extracellular signal-regulated kinases 1/2 (ERK1/2) are involved in osteogenesis and adipogenesis, the aim of our study was to investigate if the effects of Ti surface topography on osteoblast and adipocyte differentiation are modulated by ERK1/2. The experiments were conducted based on the effect of the ERK1/2 inhibitor, PD98059, on mesenchymal stem cells (MSCs) grown under osteogenic and adipogenic conditions on Ti with nanotopography (Ti-Nano) or on machined Ti (Ti-Machined). The results showed that, in general, ERK1/2 inhibition favored osteoblast and adipocyte differentiation of MSCs grown on Ti-Machined. In MSCs grown on Ti-Nano, ERK1/2 inhibition upregulated the expression of alkaline phosphatase and osteocalcin and reduced extracellular matrix mineralization. In terms of adipocyte differentiation, ERK1/2 inhibition elicited similar MSC responses to Ti-Nano and Ti-Machined, upregulating gene expression of adipocyte markers without affecting lipid accumulation. Our results indicate that, under osteogenic and adipogenic conditions, the responses of MSCs to Ti surface topography in terms of osteogenesis and adipogenesis are dependent on ERK1/2. Thus, a precise modulation of ERK1/2 expression and activity induced by surface topography could be a good strategy to drive the process of implant osseointegration. (AU)

• Processo FAPESP: 16/00182-0 - Participação de auinases de adesão focal na diferenciação osteoblástica de células-tronco mesenquimais induzida por superfícies de titânio com micro e nanotopografia
• Beneficiário: Marcelo Soares Francischini
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/01291-6 - Participação das proteínas ósseas morfogenéticas 2 e 4 endógenas na expressão do fenótipo osteoblástico em células crescidas sobre titânio com nanotopografia
• Beneficiário: Márcio Mateus Beloti
• Modalidade de apoio: Auxílio à Pesquisa - Regular