| Texto completo | |
| Autor(es): |
Valenzuela-Oses, Johanna K.
;
Garcia, Monica C.
;
Feitosa, Valker A.
;
Pachioni-Vasconcelos, Juliana A.
;
Gomes-Filho, Sandro M.
;
Lourenco, Felipe R.
;
Cerize, Natalia N. P.
;
Basseres, Daniela S.
;
Rangel-Yagui, Carlota O.
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Materials Science & Engineering C-Materials for Biological Applications; v. 81, p. 327-333, DEC 1 2017. |
| Citações Web of Science: | 8 |
| Resumo | |
Miltefosine presents antineoplastic activity but high hemolytic potential. Its use in cancer has been limited to treating cutaneous metastasis of breast cancer. To decrease hemolytic potential, we developed a formulation of miltefosine-loaded polymeric micelles (PM) of the copolymer Pluronic-F127. A central composite design was applied and the analysis of variance showed that the optimum level of hydrodynamic diameter and poly-dispersity index predicted by the model and experimentally confirmed were 29 nm and 0.105, respectively. Thermal analyses confirmed that miltefosine was molecularly dispersed within PM. Pluronic-F127 PM with miltefosine 80 mu M presented a significant reduction of hemolytic effect (80%, p < 0.05) in comparison to free drug. In vitro assays against HeLa carcinoma cells demonstrated similar cytotoxicity to free miltefosine and PM. Our results suggest that, by lowering hemolytic potential, miltefosine-loaded Pluronic-F127 PM a promising alternative to broaden this drug use in cancer therapy, as well as of other alkylphosphocholines. (AU) | |
| Processo FAPESP: | 14/01983-0 - Síntese e desenvolvimento nanotecnológico de análogos homocolínicos da miltefosina |
| Beneficiário: | Carlota de Oliveira Rangel Yagui |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 10/52685-9 - Novos alvos terapêuticos no câncer de pulmão associado a mutações no oncogênese K-Ras |
| Beneficiário: | Daniela Sanchez Basseres |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |