Identification of hispidin as a bioluminescent active compound and its recycling biosynthesis in the luminous fungal fruiting body

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Oba, Yuichi ; Suzuki, Yoshiki ; Martins, Gabriel N. R. ; Carvalho, Rodrigo P. ; Pereira, Tatiana A. ; Waldenmaier, Hans E. ; Kanie, Shusei ; Naito, Masashi ; Oliveira, Anderson G. ; Dorr, Felipe A. ; Pinto, Ernani ; Yampolsky, Ilia V. ; Stevani, Cassius V.

• Tipo de documento: Artigo Científico
• Fonte: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 16, n. 9, p. 1435-1440, SEP 1 2017.
• Citações Web of Science: 6

Resumo

We previously showed that luminous fungi share a common mechanism in bioluminescence, and identified hispidin as a luciferin precursor in Neonothopanus nambi mycelium. Here we showed the presence of hispidin as a bioluminescent active compound at 25-1000 pmol g(-1) in the fruiting bodies of Mycena chlorophos, Omphalotus japonicus, and Neonothopanus gardneri. These results suggest that luminous mushrooms contain hispidin as a luciferin precursor. We also found that non-luminous ``young{''} fruiting bodies exhibited luminescence by hispidin treatment. Furthermore, we observed a gradual luminescence enhancement of the cell-free fruiting body extract by the addition of hispidin biosynthetic components, namely caffeic acid, ATP and malonyl-CoA. These findings suggest that continuous weak glow of luminous mushrooms is regulated by slow recycling biosynthesis of hispidin. (AU)

• Processo FAPESP: 10/11578-5 - Purificação e clonagem da redutase e luciferase de fungos bioluminescentes.
• Beneficiário: Anderson Garbuglio de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/16885-1 - Bioluminescência em fungos: levantamento de espécies, estudo mecanístico & ensaios toxicológicos
• Beneficiário: Cassius Vinicius Stevani
• Modalidade de apoio: Auxílio à Pesquisa - Regular