| Texto completo | |
| Autor(es): Mostrar menos - |
Oba, Yuichi
;
Suzuki, Yoshiki
;
Martins, Gabriel N. R.
;
Carvalho, Rodrigo P.
;
Pereira, Tatiana A.
;
Waldenmaier, Hans E.
;
Kanie, Shusei
;
Naito, Masashi
;
Oliveira, Anderson G.
;
Dorr, Felipe A.
;
Pinto, Ernani
;
Yampolsky, Ilia V.
;
Stevani, Cassius V.
Número total de Autores: 13
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 16, n. 9, p. 1435-1440, SEP 1 2017. |
| Citações Web of Science: | 6 |
| Resumo | |
We previously showed that luminous fungi share a common mechanism in bioluminescence, and identified hispidin as a luciferin precursor in Neonothopanus nambi mycelium. Here we showed the presence of hispidin as a bioluminescent active compound at 25-1000 pmol g(-1) in the fruiting bodies of Mycena chlorophos, Omphalotus japonicus, and Neonothopanus gardneri. These results suggest that luminous mushrooms contain hispidin as a luciferin precursor. We also found that non-luminous ``young{''} fruiting bodies exhibited luminescence by hispidin treatment. Furthermore, we observed a gradual luminescence enhancement of the cell-free fruiting body extract by the addition of hispidin biosynthetic components, namely caffeic acid, ATP and malonyl-CoA. These findings suggest that continuous weak glow of luminous mushrooms is regulated by slow recycling biosynthesis of hispidin. (AU) | |
| Processo FAPESP: | 10/11578-5 - Purificação e clonagem da redutase e luciferase de fungos bioluminescentes. |
| Beneficiário: | Anderson Garbuglio de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 13/16885-1 - Bioluminescência em fungos: levantamento de espécies, estudo mecanístico & ensaios toxicológicos |
| Beneficiário: | Cassius Vinicius Stevani |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |