Identification of hispidin as a bioluminescent active compound and its recycling biosynthesis in the luminous fungal fruiting body

Author(s):Show less -

Oba, Yuichi ; Suzuki, Yoshiki ; Martins, Gabriel N. R. ; Carvalho, Rodrigo P. ; Pereira, Tatiana A. ; Waldenmaier, Hans E. ; Kanie, Shusei ; Naito, Masashi ; Oliveira, Anderson G. ; Dorr, Felipe A. ; Pinto, Ernani ; Yampolsky, Ilia V. ; Stevani, Cassius V.

• Document type: Journal article
• Source: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 16, n. 9, p. 1435-1440, SEP 1 2017.
• Web of Science Citations: 6

Abstract

We previously showed that luminous fungi share a common mechanism in bioluminescence, and identified hispidin as a luciferin precursor in Neonothopanus nambi mycelium. Here we showed the presence of hispidin as a bioluminescent active compound at 25-1000 pmol g(-1) in the fruiting bodies of Mycena chlorophos, Omphalotus japonicus, and Neonothopanus gardneri. These results suggest that luminous mushrooms contain hispidin as a luciferin precursor. We also found that non-luminous ``young{''} fruiting bodies exhibited luminescence by hispidin treatment. Furthermore, we observed a gradual luminescence enhancement of the cell-free fruiting body extract by the addition of hispidin biosynthetic components, namely caffeic acid, ATP and malonyl-CoA. These findings suggest that continuous weak glow of luminous mushrooms is regulated by slow recycling biosynthesis of hispidin. (AU)

• FAPESP's process: 10/11578-5 - Purification and cloning of a reductase and luciferase from bioluminescent fungi.
• Grantee: Anderson Garbuglio de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/16885-1 - Fungal bioluminescence: species survey, mechanistic study & toxicological assays
• Grantee: Cassius Vinicius Stevani
• Support Opportunities: Regular Research Grants