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Fungal bioluminescence: species survey, mechanistic study & toxicological assays

Grant number: 13/16885-1
Support type:Regular Research Grants
Duration: December 01, 2013 - September 30, 2016
Field of knowledge:Biological Sciences - Biophysics
Principal Investigator:Cassius Vinicius Stevani
Grantee:Cassius Vinicius Stevani
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Over the last half decade the study of fungal bioluminescence has regained momentum since the involvement of enzymes in the cell free in vitro bioluminescent reaction has been confirmed after over 40 years of controversy. Since then our laboratory has worked mainly on further characterizing the substances involved in fungal bioluminescence and its mechanism, as well as on the development of a toxicological bioluminescent assay with fungi. Previously, we proved the involvement of a NAD(P)H-dependent reductase and a membrane-bound luciferase in a two-step reaction triggered by addition of NAD(P)H and molecular oxygen to generate green light. The fungal luminescent system is also likely shared across all lineages of bioluminescent fungi based on cross-reaction studies. Moreover, fungal bioluminescence is inhibited by the mycelium exposure to toxicants. The change in light emission under optimal and controlled conditions has been used as endpoint to the development of toxicological bioassays. These bioassays are useful to better understand the interactions and effects of hazardous compounds to terrestrial species and to assist the assessment of soil contaminations by biotic or abiotic sources. In this work, we present an overview of the current state of the study of fungal luminescence and the application of bioluminescent fungi as versatile tool in toxicology. (AU)

Articles published in Agência FAPESP Newsletter about the research grant
First South American insect that emits blue light is discovered 
Articles published in Pesquisa FAPESP Magazine about the research grant:
Natural lighting 

Scientific publications (10)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FALASCHI, RAFAELA L.; AMARAL, DANILO T.; SANTOS, ISAIAS; DOMINGOS, ADAO H. R.; JOHNSON, GRANT A.; MARTINS, ANA G. S.; VIROOMAL, IMRAN B.; POMPEIA, SERGIO L.; MIRZA, JEREMY D.; OLIVEIRA, ANDERSON G.; BECHARA, ETELVINO J. H.; VIVIANI, VADIM R.; STEVANI, V, CASSIUS. Neoceroplatus betaryiensis nov. sp. (Diptera: Keroplatidae) is the first record of a bioluminescent fungus-gnat in South America. SCIENTIFIC REPORTS, v. 9, AUG 5 2019. Web of Science Citations: 0.
RAFAELA LOPES FALASCHI; GRANT A. JOHNSON; CASSIUS VINICIUS STEVANI. The new species Neoceroplatus betaryiensis nov. sp. (Diptera: Keroplatidae) from Neotropical Region. Papéis Avulsos de Zoologia (São Paulo), v. 59, p. -, 2019.
KOTLOBAY, ALEXEY A.; SARKISYAN, KAREN S.; MOKRUSHINA, YULIANA A.; MARCET-HOUBEN, MARINA; SEREBROVSKAYA, EKATERINA O.; MARKINA, NADEZHDA M.; SOMERMEYER, LOUISA GONZALEZ; GOROKHOVATSKY, ANDREY Y.; VVEDENSKY, ANDREY; PURTOV, KONSTANTIN V.; PETUSHKOV, VALENTIN N.; RODIONOVA, NATALJA S.; CHEPURNYH, TATIANA V.; FAKHRANUROVA, LILIIA I.; GUGLYA, ELENA B.; ZIGANSHIN, RUSTAM; TSARKOVA, ALEKSANDRA S.; KASKOVA, ZINAIDA M.; SHENDER, VICTORIA; ABAKUMOV, MAXIM; ABAKUMOVA, TATIANA O.; POVOLOTSKAYA, INNA S.; EROSHKIN, FEDOR M.; ZARAISKY, ANDREY G.; MISHIN, ALEXANDER S.; DOLGOV, SERGEY V.; MITIOUCHKINA, TATIANA Y.; KOPANTZEV, EUGENE P.; WALDENMAIER, HANS E.; OLIVEIRA, ANDERSON G.; OBA, YUICHI; BARSOVA, EKATERINA; BOGDANOVA, EKATERINA A.; GABALDON, TONI; STEVANI, CASSIUS V.; LUKYANOV, SERGEY; SMIRNOV, IVAN V.; GITELSON, JOSEF I.; KONDRASHOV, FYODOR A.; YAMPOLSKY, ILIA V. Genetically encodable bioluminescent system from fungi. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 115, n. 50, p. 12728-12732, DEC 11 2018. Web of Science Citations: 4.
OBA, YUICHI; SUZUKI, YOSHIKI; MARTINS, GABRIEL N. R.; CARVALHO, RODRIGO P.; PEREIRA, TATIANA A.; WALDENMAIER, HANS E.; KANIE, SHUSEI; NAITO, MASASHI; OLIVEIRA, ANDERSON G.; DORR, FELIPE A.; PINTO, ERNANI; YAMPOLSKY, ILIA V.; STEVANI, CASSIUS V. Identification of hispidin as a bioluminescent active compound and its recycling biosynthesis in the luminous fungal fruiting body. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 16, n. 9, p. 1435-1440, SEP 1 2017. Web of Science Citations: 6.
KASKOVA, ZINAIDA M.; DORR, FELIPE A.; PETUSHKOV, VALENTIN N.; PURTOV, KONSTANTIN V.; TSARKOVA, ALEKSANDRA S.; RODIONOVA, NATALJA S.; MINEEV, KONSTANTIN S.; GUGLYA, ELENA B.; KOTLOBAY, ALEXEY; BALEEVA, NADEZHDA S.; BARANOV, MIKHAIL S.; ARSENIEV, ALEXANDER S.; GITELSON, JOSEF I.; LUKYANOV, SERGEY; SUZUKI, YOSHIKI; KANIE, SHUSEI; PINTO, ERNANI; DI MASCIO, PAOLO; WALDENMAIER, HANS E.; PEREIRA, TATIANA A.; CARVALHO, RODRIGO P.; OLIVEIRA, ANDERSON G.; OBA, YUICHI; BASTOS, ERICK L.; STEVANI, CASSIUS V.; YAMPOLSKY, ILIA V. Mechanism and color modulation of fungal bioluminescence. SCIENCE ADVANCES, v. 3, n. 4 APR 2017. Web of Science Citations: 19.
OBA, YUICHI; STEVANI, CASSIUS V.; OLIVEIRA, ANDERSON G.; TSARKOVA, ALEKSANDRA S.; CHEPURNYKH, TATIANA V.; YAMPOLSKY, ILIA V. Selected Least Studied but not Forgotten Bioluminescent Systems. Photochemistry and Photobiology, v. 93, n. 2, p. 405-415, MAR-APR 2017. Web of Science Citations: 9.
DESJARDIN, DENNIS E.; PERRY, BRIAN A.; STEVANI, CASSIUS V. New luminescent mycenoid fungi (Basidiomycota, Agaricales) from Sao Paulo State, Brazil. Mycologia, v. 108, n. 6, p. 1165-1174, NOV-DEC 2016. Web of Science Citations: 3.
BAADER, WILHELM J.; STEVANI, CASSIUS V.; BECHARA, ETELVINO J. H. ``Photo{''} Chemistry Without Light?. Journal of the Brazilian Chemical Society, v. 26, n. 12, p. 2430-2447, DEC 2015. Web of Science Citations: 8.
OLIVEIRA, ANDERSON G.; STEVANI, CASSIUS V.; WALDENMAIER, HANS E.; VIVIANI, VADIM; EMERSON, JILLIAN M.; LOROS, JENNIFER J.; DUNLAP, JAY C. Circadian Control Sheds Light on Fungal Bioluminescence. Current Biology, v. 25, n. 7, p. 964-968, MAR 30 2015. Web of Science Citations: 25.
WILHELM J. BAADER; CASSIUS V. STEVANI; ETELVINO J. H. BECHARA. "Photo" Chemistry Without Light?. Journal of the Brazilian Chemical Society, v. 26, n. 12, p. -, Dez. 2015.

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