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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of Phenolic Compound Toxicity Using a Bioluminescent Assay with the Fungus Gerronema viridilucens

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Author(s):
Ventura, Fernanda F. [1, 2] ; Mendes, Luiz F. [1, 3] ; Oliveira, Anderson G. [4] ; Bazito, Reinaldo C. [1] ; Bechara, Etelvino J. H. [1] ; Freire, Renato S. [1] ; Stevani, V, Cassius
Total Authors: 7
Affiliation:
[1] V, Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, Sao Paulo, SP - Brazil
[2] Fundacao Jorge Duprat Figueiredo Seguranca & Med, Sao Paulo, SP - Brazil
[3] Bioat Grp, Santana Do Parnaiba, SP - Brazil
[4] Univ Sao Paulo, Inst Oceanog, Dept Oceanog Fis Quim & Geol, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Environmental Toxicology and Chemistry; v. 39, n. 8 JUN 2020.
Web of Science Citations: 0
Abstract

Basidiomycetes (phylum Basidiomycota) are filamentous fungi characterized by the exogenous formation of spores on a club-shaped cell called a basidium that are often formed on complex fruiting bodies (mushrooms). Many basidiomycetes serve an important role in recycling lignocellulosic material to higher trophic levels, and some show symbiotic relationships with plants. All known bioluminescent fungi are mushroom-forming basidiomycetes in the order Agaricales. Hence, the disruption of the basidiomycete community can entirely compromise the carbon cycle in nature from fungi to higher trophic levels. The fungus Gerronema viridilucens was used in the present study to investigate the toxicity of a phenolic compound series based on the inhibition of its bioluminescence. The median effect concentration (EC50) obtained from curves of bioluminescence inhibition versus log {[}phenolic compound] showed that 2,4,6-trichlorophenol was the most toxic compound in the series. The log EC50 values of all phenolic compounds were then used for the prediction of their toxicity. The univariate correlation of log EC50 values obtained from 6 different phenolic compounds was stronger with the dissociation constant (pK(a)) than with 1-octanol/water partition coefficient (K-OW). Nevertheless, the toxicity can be better predicted by using both parameters, suggesting that the phenol-driven uncoupling of fungus mitochondrial adenosine triphosphate synthesis is the origin of phenolic compound toxicity to the test fungus. Environ Toxicol Chem 2020;00:1-8.(c) 2020 SETAC (AU)

FAPESP's process: 17/22501-2 - Electronic chemiexcitation in biological systems: bioluminescence and photochemistry in the dark
Grantee:Etelvino José Henriques Bechara
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/16885-1 - Fungal bioluminescence: species survey, mechanistic study & toxicological assays
Grantee:Cassius Vinicius Stevani
Support type: Regular Research Grants