Temperature, pH and carbon source affect drastically indole acetic acid production of plant growth promoting yeasts

Autor(es)

Ana Silvia de Almeida Scarcella ^[1] ; Rodolfo Bizarria Junior ^[2] ; Reinaldo Gaspar Bastos ^[3] ; Márcia Maria Rosa Magri ^[4]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Department of Biochemistry and Immunology - Brasil

^[2] Universidade Federal de São Carlos. Centro de Ciências Agrárias - Brasil

^[3] Universidade Federal de São Carlos. Centro de Ciências Agrárias - Brasil

^[4] Universidade Federal de São Carlos. Centro de Ciências Agrárias - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Chemical Engineering; v. 34, n. 2, p. 429-438, 2017-04-00.

Resumo

Abstract Rhizosphere and phylloplane yeasts are able to produce phytohormones, acting as potential plant growth promoters. The aim of this study was to analyze the production of indole acetic acid (IAA) by yeasts using variations in the culture conditions. The yeasts evaluated were Rhodotorula mucilaginosa and Trichosporon asahii, which were cultivated in a potato broth medium with 20 g/L of glucose or sucrose as the carbon source. The cultivation was carried out at 22°C, 25°C or 30°C, and the initial pH was adjusted to 3.0, 4.5 or 6.0. The cultures were analyzed using a colorimetric technique with Salkowski reagent. The cell count was determined in all of the conditions. The results for T. asahii showed that IAA production with sucrose was superior at pH 6.0, and that with glucose was superior at pH 4.5. These results indicate an association between the carbon source and medium pH. Rh. mucilaginosa (2F32) presented a high IAA production when compared with T. asahii (3S44 and 4C06) or even with other microorganisms referenced in the literature. The highest production (0.655 g/L) was obtained at pH 6.0 with glucose as the carbon source. Variations in the culture conditions did not affect the cell growth of the yeasts. (AU)

• Processo FAPESP: 11/02322-0 - Caracterização de leveduras isoladas de áreas agrícolas como promotoras de crescimento vegetal e na interação com fungos micorrízicos e rizobactérias
• Beneficiário: Márcia Maria Rosa Magri
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/02421-3 - Avaliação da interação de leveduras promotoras de crescimento vegetal com fungos micorrízicos e rizobactérias
• Beneficiário: Rodolfo Bizarria Júnior
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/18676-5 - Caracterização de leveduras isoladas de áreas agrícolas na promoção de crescimento vegetal.
• Beneficiário: Ana Sílvia de Almeida Scarcella
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico