Metal toxicity assessment by sentinel species of mangroves: In situ case study integrating chemical and biomarkers analyses

Autor(es)

de Almeida Duarte, Luis Felipe ^[1] ; de Souza, Caroline Araujo ^[1] ; Seabra Pereira, Camilo Dias ^{[2, 3]} ; Amaro Pinheiro, Marcelo Antonio ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Inst Biociencias IB, Lab Biol Crustaceos, Grp Pesquisa Biol Crustaceos CRUSTA), Campus Litoral Paulista CLP, BR-11330900 Sao Paulo - Brazil

^[2] UNIFESP Univ Fed Sao Paulo, Dept Ciencias Mar, Campus Baixada Santista, BR-11030400 Santos, SP - Brazil

^[3] Univ Santa Cecilia, Lab Ecotoxicol, UNISANTA, Rua Oswado Cruz 266, BR-11045900 Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 145, p. 367-376, NOV 2017.
• Citações Web of Science: 7

Resumo

Globally, there is a lack of knowledge about tropical ecotoxicology dealing with the potential impact of metal contamination in mangrove ecosystem. This habitat is considered a nursery for several animal species, among them the ``uca{''}-crab (Ucides cordatus), known as a key species due to its biological and economical importance. This study evaluated the association involving metal contamination (Cd, Cu, Pb, Cr, Mn and Hg) in water, sediment, red-mangrove vegetation (Rhizophora mangle) and tissues of uga crab, together with its geno-cytotoxic responses, based on micronucleated hemocytes frequency and the retention time of neutral red in lysosomes. We assessed six mangrove areas with distinct pollution levels in Sao Paulo State, Brazil, where the water and sediment contamination by metals were associated with accumulation of these pollutants in biotic compartments (mangrove leaves and crab). In U. cordatus, metal accumulation was best explained by metal concentration found in leaves of R. mangle than in the water or sediment, indicating that feeding drives metal exposure in this organism. Mercury (Hg) concentration in sediment, copper (Cu) concentration in hepatopancreas of U. cordnnic and lead (Pb) in water and green leaves of R. mangle showed a significant correlation with genotoxic impact in U. cordatus. However, copper concentration (in green/senescent leaves and hepatopancreas) and lead (in sediment), were the major metals affecting lysosomal membrane integrity. Therefore, representatives of all compartments were associated with cyto and genotoxicity in this species, thus requiring a holistic approach to issues related to sublethal damage. Probability estimates of cytogenetic impacts related to metal concentration in abiotic compartments (significantly correlated with known biomarkers: Hg in sediment; and Pb in water and sediment) are also presented. Our results highlight the need for environmental restoration of mangroves areas contaminated with metals, responsible for cytogenetic injuries and revealing a pre-pathological condition in this sentinel species, in addition to ecological disturbances. (AU)

• Processo FAPESP: 09/14725-1 - Projeto Uçá III - Impacto genotóxico em populações do caranguejo-uçá, Ucides cordatus (Linnaeus, 1763) (Crustacea, Brachyura, Ucididae): avaliação e correlação com a concentração de metais pesados em cinco manguezais do estado de São Paulo
• Beneficiário: Marcelo Antonio Amaro Pinheiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/01552-9 - Impacto genotóxico em populações do caranguejo-uçá, Ucides cordatus (Linnaeus, 1763)(Crustacea, Brachyura, Ucididae), em cinco manguezais do Estado de São Paulo, Brasil.
• Beneficiário: Luis Felipe de Almeida Duarte
• Modalidade de apoio: Bolsas no Brasil - Doutorado